Abstract: Objective To investigate the effects of ursolic acid on the expression of HMGB1 and activity of NF-κB in THP-1 cells. Methods After being stimulated by different concentrations UA (0,0.1,1,5 and 10μmol/L), the cells were detected correspondingly.⑴The cell proliferation were checked by MTT. ⑵The HMGB1 and p65 destination genes were amplificated with PCR.⑶HMGB1 and p65 protein was detected by Western blot. ⑷Transfecting of Plasmid pGl3-NF-кB-luciferase report gene:Plasmid pGl3-NF-кB-luciferase report gene was transient transfected into THP-1 cells.After transfecting for 40 hours, 1 μmol/L UA were added. Luciferase activity detection was performed when transfecting for 48 hours. Results ⑴UA can obviously inhibit THP-1 cell proliferation with the concentration increasing.⑵The influence of UA on THP-1 cell HMGB1 mRNA and protein exhibits a certain bidirectional and the influence of 1 μmol/L UA is the strongest.⑶UA can influence the expression of p65 mRNA and protein andaffect the NF-кBactivity.Conclusion UA can influence the HMGB1 expression and NF-кB activity of THP-1 cell.

Key words: Ursolic acid, THP-1, HMGB1, NF-кB