Abstract: Objective This study aimed to investigate effects of RON on proliferation and migration in T24 cell line. Methods First, RT-PCR was used to detect expression of RON mRNA in bladder cancer tissues. Second, Knockdown of RON expression in T24 cell line was conducted by transfection of a chemical synthesized small interfering RNA specifically targeted to RON. Efficacy of RON-silenced was determined ?by real time -PCR and western blot, The CCK-8, Transwell migration and invasion assays were performed to measure the proliferation, migration and invasion abilities, respectively. Flow cytometry was adopted to detect cell apoptosis. Results The results showed that the RON mRNA relative expression level was positive correlation with TNM stage; in RON-siRNA group, cell proliferation and migration abilities were degraded (P<0.05), and cell apoptosis was increased (P<0.05), compared with other groups. Conclusion RON gene promoted proliferation and migration of bladder cancer cells, maybe become a new target for the treatment of bladder cancer.

Key words: small interfering RNA, bladder cancer, T24 cells, MST1R, RON