Basic & Clinical Medicine ›› 2014, Vol. 34 ›› Issue (5): 579-582.

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Generation of induced neural stem/progenitor cells from human fibroblasts

  

  • Received:2013-12-26 Revised:2014-01-07 Online:2014-05-05 Published:2014-04-28
  • Contact: Y.Alex Zhang E-mail:zhangyualex1975@tom.com

Abstract: Objective To investigate induced neural stem/progenitor cells (iNSCs) from human fibroblasts. Methods Human fibroblasts were infected with lenti-virus expressing green fluorescent protein (GFP) under Pax6 promoter, followed by a hygromycin-resistant gene. Hygromycin-selected fibroblasts were used as starter cells and infected with virus encoding 10 transcription factors Sox2, Klf4, c-Myc, Tcf3, Ascl1, Brn2, Neurog2, Foxg1, Hes1, Id1, which play critical roles in the development, differentiation, functional stability, and plasticity of neural cells. The reprogrammed cells that were GFP-positive were sorted by using flow cytometer and characterized by fluorescence staining and differentiation assay. Results iNSC expressed NSC markers (Nestin) and could differentiate into neurons(β Ⅲ- tubulin) and astrocytes(GFAP). Conclusion Human fibroblasts can be converted to NSC-likes cells by 10 transcription factors.

Key words: Key words: induced neural stem/progenitor cells, human fibroblast, reprogramming, transcription factors, pax6 reporter gene

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