Abstract: Dorsal root ganglion neurons(DRGn) play an important role in diabetic peripheral neuropathy. Currently, the effects of high glucose on DRGn in vitro has become a research hotspot. High glucose result in apoptosis of DRGn. Moreover, DRGn showed differences in oxidative stress, activation of membrane receptor mGluRs and TRPV1, DRGn/SCs co-cultures between in high glucose and in defined medium.