Abstract: Objective To explore the effect of the combination of small dosage of VCR and Endostar on rhabdomyosarcoma cells and its mechanisms. Methods PLA-802 was cultured and treated with small dosage of VCR or Endostar singly or together. MTT was used to study the cells proliferation, and flow cytometry was for the cell cycle. While, RT-PCR and Western blot were for the expression of VEGF mRNA and protein levels. ELISA was for the generation of VEGF in supernatant. Results VCR (0.5 and 2.0μg/ml) or Endo (2.0 μg/ml) used singly inhibited the cell proliferation and promoted the apoptosis, and inhibited the expression and generation of VEGF. When the cells were treated with VCR and Endo together, the inhibitory effects of them were significantly strengthened. Conclusion The combination of VCR and Endostar could synergistically inhibit the expression and generation of VEGF and inducing the apoptosis in PLA-802 cells.