Basic & Clinical Medicine ›› 2009, Vol. 29 ›› Issue (2): 144-147.

• 研究论文 • Previous Articles     Next Articles

Repairing on Rabbit Facial Nerve by the Transplanted of NSCs- HA-NT-3 Complex

Han ZHANG, Yue-teng WEI, Chong-ran SUN, Jin LI, Hua HUANG, Fu-zhai CUI, Yi-hua AN   

  1. Capital Medical University,Beijing Neurosurgical Institute Capital Medical University,Beijing Neurosurgical Institute
  • Received:2008-01-08 Revised:2008-03-27 Online:2009-02-25 Published:2009-02-25
  • Contact: Yi-hua AN

Abstract: Objective This experiment explored the possibility of repairing injured facial nerve with tissue engineering technology and neural stem cells(NSCs). The complex consisted of NSCs, scaffold and NT-3. NSCs were immature cells with the potential of self-renewal and multiple differentiation to neurons and glial cells. The scaffold with porous surface was made of hyaluronic acid and collagen (HA-Col gel) which degenerate in vivo after transplantation. NT-3 is the signal proved effective in promoting neurons survival in vitro. Methods NSCs of S-D rat were co-cultured with scaffold and NT-3 in vitro. The two stumps of disconnected facial nerve of rabbit were re-connected with the complex. Electrophysiology and morphology tests were used to examine the functional and morphological changes.Results NSCs adhered to the HA-Col gel and survived. Injured facial nerve fixed by NSCs-HA-Col gel-NT-3 complex showed significant improvement in function and anatomical structure. Conclusion The result demonstrated that combinative implant of NSCs, HA-Col gel and NT-3 could promote the regeneration of injured facial nerve.

Key words: neural stem cells, tissue engineering, regeneration