Abstract: Objective To explore one of the possible signal transduction pathways of FKN/CX3CR1 in human peripheral blood monocytes (PBMC) and the mechanism of FKN in promoting atherosclerosis,the function of protein kinase C is also studied.Methods （1）Human peripheral blood monocytes were isolated from fresh blood of healthy volunteers by Ficoll-Paque gradient centrifugation.（2）Divided the PBMC into four groups:control,FKN,Ro31-8220 and PD98059 groups.（3）Measured the ERK 1/2 expression of monocytes from each group by Western blot.（4）Collected the supernatant of monocytes from each group ,determined the expression of TNF-α by enzyme-linked immunosorbent assay(ELISA).Results （1）The expression of ERK1/2 and TNF－α from FKN group was significantly higher compared with the control and TNF－α from FKN group was significantly higher compared with the control group（P<0.05）.（2）The expression of ERK1/2 and TNF－α from Ro31-8220 group was decreased compared with the FKN group（P<0.05）.Conclusions FKN－CX3CR1 induces the expression of TNF－α via PKC/ERK signal pathway which contributines to the development and progression of atherosclerosis.