Basic & Clinical Medicine ›› 2009, Vol. 29 ›› Issue (11): 1144-1149.

• 研究论文 • Previous Articles     Next Articles

The effects of human S100A6 on β-catenin in two human osteosarcoma cell lines

Jia WEI, Ying-hua CHEN, Li-mei WU, Wen MA, Yan ZHANG, Tong-chuan HE, Lan ZHOU   

  1. Chongqing Medical University Chongqing Medical University
  • Received:2008-12-29 Revised:2009-04-07 Online:2009-11-20 Published:2009-11-20
  • Contact: Lan ZHOU

Abstract: Objective To investigate the effects of the human S100A6 on β-catenin in human osteosarcoma cell lines MG63 and U2OS . Methods Cell lines MG63 and U2OS were infected by recombinant adenoviruses carrying the human S100A6 and its siRNA gene, AdS100A6 and AdSiS100A6, respectively, to up-regulate and down-regulate the expression of S100A6. Then RT-PCR,Western blot and immunocytochemistry were used to detect mRNA and protein (level and/or distribution ) of β-catenin. Results In both cell lines MG63 and U2O S, with up-re gulation of S100A6, the expression of β-catenin mRNA and protein increased(P<0.05)and β-catenin protein increase was more obvious in nuclear than in cytoplasma; while down-regulating S100A6, both the mRNA and protein level of β-catenin decreased(P<0.05); β-catenin protein decrease was more obvious in nuclear than in cytoplasma, too. Conclusion. Increaseing Wnt/β-catenin signaling activity may be a mechanism that S100A6 involves in tumor development.