Abstract: Objective To establish the acute hind limb ischemia on Wistar rat with diabetes mellitus. Methods 30 male Wistar rats were induced by intraperitoneal STZ (50mg/kg) injection, as well as the blood glucose level tested over 16.8mmol/L. The rats were ligated on the left femoral artery, then the blood perfusion on the hind limbs ischemia were measured by LDPI after the operation. Results The fasting plasma glucose level on 22 Wistar rats(81.5%) were kept above 16.8mmol/L, and the hind limb blood perfusion would recover slowly to the level of the right side from 1 to 14 day (p<0.05). There is no distinctly significance on both sides until the 21st day. Conclusion Followed by the above mentioned protocol, the acute hind limb ischemia on the diabetic Wistar rat can be established during 3 weeks, which provided the possibility to investigate the healing mechanism of the diabetic food ulcer.