Abstract: Objective To investigate the effects of glycemic control on myocardial glucose metabolism in type 2 diabetic rats．Methods Eighteen male Sprague-Dawley rats were randomized into three groups：control, diabetes, therapy．Fed with high一fat diet and intraperitoneally injected with streptozotocin to establish type 2 diabetic model，the rate of carbohydrate oxidation was measured by isolated heart Langendorf perfusion apparatus, the expression level of glucose transport 4(GLUT4) was measured by Western blotting. Results Compared with the control group，the diabetic rats had a significantly depression of glucose uptake in the hearts（P<0．O1）. The oxidation rate of glucose decreased , and the oxidation rate of free fatty acid increased (P<0．05). The expression level of GLUT4 in myocardium was decreased by 53％ ．When compared with the diabetic rats, in insulin-treated rats ,glucose uptake and the rate of glucose oxidation in myocardium was increased, the rate of fatty acid oxidation was decreased, and the expression level of GLUT4 was increased by 47％ (P<0．01)．Conclusions Glycemic control improved myocardial glucose metabolism in diabetic rats.