Abstract: Abstract: Objective To investigate the expression and relationship of P27kip1 and nuclear export factor CRM1 in lymphoma cells. Methods Cells were synchronized using serum starvation, 10%serum stimulating proliferation. The expression of P27kip1 and Jab1 were detected by cell fractionation and Western blot. LMB were used for stimulating Jurkat cells those were in proliferation process, and then detect the expression of P27kip1 and Jab1. Using immunoprecipitation detect the combination of P27kip1 and CRM1. Results The albumen amount of P27kip1 was always contrary with the albumen amount of CRM1. What’s more, the location of P27kip1 accorded with the expression of CRM1. P27kip1 and CRM1 could form compound in Jurkat and Raji cells detected by immunoprecipitation. Conclusion These indicated the CRM1 may influence the location and expression of P27kip1 through integrating with P27kip1, and then may participate in regulating the growth of NHL cells.

Key words: P27kip1, CRM1, export, Lymphoma