Basic & Clinical Medicine ›› 2008, Vol. 28 ›› Issue (12): 1251-1254.
• 研究论文 • Previous Articles Next Articles
Jing CHANG, Ling-qiao LU, Hong-xia WANG, Jing WANG, Li-quan MA, Shao-peng ZHENG, Li-ke ZHANG
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Abstract: Objective To detect CYP2J3 gene expression and contents of 11,12-EET in heart, liver, lung, kidney and aorta thoracalis after CYP2J3 gene transfection . Methods The rat transgenic model was built by injecting plasmid through vena dorsalis penis. The animals were divided into control group、 pcDNA3.1 transgenic group and pcDNA3.1- CYP2J3 transgenic group. The expression of CYP2J3 mRNA was detected by RT-PCR method and content of 11,12-EET was examined by the HPLC in heart, liver, lung, kidney and aorta thoracalis at 14 days and 28 days after injection. Results At 28 days after injection, the expression of CYP2J3 mRNA and the content of 11,12-EET were significantly increased than that of control and pcDNA3.1 transgenic group ( P<0.05) . Conclusions The method of injecting plasmid can increase the expression of CYP2J3 in heart, liver, lung, kidney and aorta thoracalis.
Key words: CYP450, CYP2J3, plasmid, gene transfection, EETs
CLC Number:
R541.4
Jing CHANG; Ling-qiao LU; Hong-xia WANG; Jing WANG; Li-quan MA; Shao-peng ZHENG; Li-ke ZHANG. Distribution of CYP2J3 in Gene Transfection Rats by vena dorsalis penis[J]. Basic & Clinical Medicine, 2008, 28(12): 1251-1254.
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http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2008/V28/I12/1251