Abstract: Objective: To investigate the survival, differentiation and action of neural stem cell transplanted into striatum of Parkinson's disease rat model. Methods: Neural stem cells were separated and cultured in vitro. The character of self-renewing was confirmed by technique of single cell clone culture. The ability of multi-directional differentiation and specific antigen-nestin was showed by immunocytochemistry(ICC). The models of PD rat were established, and the DAPI-labelled neural stem cells were transplanted into striatum. The 6-OHDA induced rotary behavior was measured, distribution and migration of the cell were checked by fluoresce activating. ICC and HPLC are used to see its differentiation and action of secrete neurotransmitter dopamine(DA) and it's metabolites. Result: the cultured stem cell express nestin and have the ability of self-renewing and multi-directional differentiation. 6-OHDA induced rotation decreased after transplantation of NSC into the PD rat striatum（P＜0.05）。NSC migrate widely in the recipient and form small member of TH cell, fibre in striatum. Content of DA and its metabolite (dopac) of rat's striatum after transplanting increased compare with the pseudo-transplanted group. Conclusion: transplanted neural stem cell can survived in the brain of PD rat and differentiate to cells suit to microcircumstances by excreting neurotransmitter and improve the symptom of PD.