Abstract: Objective By observing the changes in NGF and its receptor P75 in liver of CCl4-induced toxic rats，to evaluate the role of NGF in the mechanism of hepatic fibrosis. Methods The expressions of NGF mRNA and its receptor P75 were detecteded by both hemi-quantitative RT-PCR and Western blot analysis in liver of CCl4-induced toxic rats at the time of 24h，48h，72h，96h、1w、5w and 9w . Results The expressions of NGFmRNA and its receptor P75 in liver of CCl4-induced toxic rats at 24th hour group were higher than that of control group （P<0.01，n=6）. Then it was swith low at 48th hour and 72th hour. But it was still higher than that of control group（P<0.05，n=6）. Conclusion In CCl4-induced rat liver tissue，the expressions of NGF and its receptor P75 might be significantly related to hepatic fibrosis.

Key words: NGF, RT-PCR, Western blot, Hepatic fibrosis, Rat