过表达miR-144-3p减轻Ang Ⅱ诱导的HUVEC损伤

doi:10.16352/j.issn.1001-6325.2023.03.444

基础医学与临床 ›› 2023, Vol. 43 ›› Issue (3): 444-449.doi: 10.16352/j.issn.1001-6325.2023.03.444

过表达miR-144-3p减轻Ang Ⅱ诱导的HUVEC损伤

叶远征^1,2, 付晓晓³, 马晓芸⁴, 范平^1,2*

新疆医科大学第一附属医院 1.心功能科;
2.省部共建中亚高发病成因与防治国家重点实验室;
4.干部特诊区,新疆维吾尔自治区乌鲁木齐 830000;
3.乌鲁木齐市中医医院药学部,新疆维吾尔自治区乌鲁木齐 830000

收稿日期:2022-06-29 修回日期:2022-10-03 出版日期:2023-03-05 发布日期:2023-02-27
通讯作者: * xjarrhyvip@163.com
基金资助:
省部共建中亚高发病成因与防治国家重点实验室开放课题项目(SKL-HIDCA-2020-7)

Over-expression of miR-144-3p attenuates Ang Ⅱ-induced injury of HUVEC

YE Yuanzheng^1,2, FU Xiaoxiao³, MA Xiaoyun⁴, FAN Ping^1,2*

1. Department of Cardiac Function;
2. State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia;
4. Cardre Ward, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830000;
3. Department of Pharmacy, Urumqi Traditional Chinese Medicine Hospital, Urumqi 830000, China

Received:2022-06-29 Revised:2022-10-03 Online:2023-03-05 Published:2023-02-27
Contact: * xjarrhyvip@163.com

摘要/Abstract

摘要： 目的探讨miR-144-3p对Ang Ⅱ诱导的人脐静脉内皮细胞(HUVEC)增殖、侵袭、凋亡和ROS水平的影响。方法将细胞分为对照组(control)、模型组(Ang Ⅱ)、阴性对照组(Ang Ⅱ+转染miR-144-3p NC)和实验组(Ang Ⅱ+转染miR-144-3p mimic)。PCR验证转染效率;MTT法检测细胞增殖;Transwell小室法检测细胞侵袭;凋亡试剂盒和流式细胞测量术检测细胞凋亡;流式细胞测量术检测细胞内活性氧(ROS)水平。结果与对照组相比,模型组细胞的增殖和侵袭能力显著下降,而凋亡率和细胞内ROS水平显著上升(P＜0.05);而实验组细胞上述指标均可得到一定程度的缓解(P＜0.05)。结论过表达miR-144-3p能够有效减轻由Ang Ⅱ诱导的HUVEC增殖、侵袭能力,以及降低Ang Ⅱ诱导的HUVEC细胞凋亡率和ROS水平。

关键词: miR-144-3p, 血管紧张素Ⅱ(Ang Ⅱ), 细胞损伤, 活性氧(ROS)

Abstract: Objective To investigate the effect of miR-144-3p on the proliferation, invasion, apoptosis and ROS levels of human umbilical vein endothelial cell (HUVEC) induced by angiotensin Ⅱ(Ang Ⅱ). Methods The cells were divided into control group, model group (Ang Ⅱ), negative control group (Ang Ⅱ+ transfection miR-144-3p NC) and experimental group (Ang Ⅱ + transfection miR-144-3p mimic). RT-qPCR was used to detect the transfection efficiency. MTT method was used to detect cell proliferation. Cell invasion was examined by Transwell chamber. Apoptosis kit and flow cytometry were applied to detect apoptosis, and the flow cytometry was applied to detect intracellular reactive oxygen species(ROS) level. Results Compared with control group, the proliferation and invasion of the cells in the model group decreased significantly, but the apoptotic rate and intracellular ROS level increased significantly(P＜0.05). The above indicators found in the experimental group were all alleviated to a certain extent (P＜0.05). Conclusions Over-expression of miR-144-3p can effectively reduce the proliferation and invasion of HUVEC induced by Ang Ⅱ and reduce the apoptosis rate as well as ROS level.

Key words: miR-144-3p, angiotensin Ⅱ(Ang Ⅱ), cell injury, reactive oxygen species(ROS)

中图分类号:

R544.1

叶远征, 付晓晓, 马晓芸, 范平. 过表达miR-144-3p减轻Ang Ⅱ诱导的HUVEC损伤[J]. 基础医学与临床, 2023, 43(3): 444-449.

YE Yuanzheng, FU Xiaoxiao, MA Xiaoyun, FAN Ping. Over-expression of miR-144-3p attenuates Ang Ⅱ-induced injury of HUVEC[J]. Basic & Clinical Medicine, 2023, 43(3): 444-449.

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过表达miR-144-3p减轻Ang Ⅱ诱导的HUVEC损伤

Over-expression of miR-144-3p attenuates Ang Ⅱ-induced injury of HUVEC

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