肺癌组织中lncRNA ZFAS1和hsa-miR-372-3p的表达与患者恶性特征及预后的关系

doi:10.16352/j.issn.1001-6325.2022.12.1861

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (12): 1861-1866.doi: 10.16352/j.issn.1001-6325.2022.12.1861

肺癌组织中lncRNA ZFAS1和hsa-miR-372-3p的表达与患者恶性特征及预后的关系

杨晔^1*, 党荣广¹, 张静¹, 董超¹, 宋雪晶²

石家庄市人民医院 1.肿瘤科一病区; 2.肿瘤科二病区,河北石家庄 050000

收稿日期:2021-12-13 修回日期:2022-05-09 出版日期:2022-12-05 发布日期:2022-11-23
通讯作者: ^* yang1986ye@163.com
基金资助:
河北省卫生健康委员会资助项目(20191462)

Correlation of expression of lncRNA ZFAS1 and hsa-miR-372-3p in lung cancer tissues with the malignant characteristics and prognosis of patients

YANG Ye^1*, DANG Rong-guang¹, ZHANG Jing¹, DONG Chao¹, SONG Xue-jing²

1. Oncology Department 1st Ward; 2. Oncology Department 2nd Ward, Shijiazhuang People's Hospital, Shijiazhuang 050000, China

Received:2021-12-13 Revised:2022-05-09 Online:2022-12-05 Published:2022-11-23
Contact: ^* yang1986ye@163.com

摘要/Abstract

摘要： 目的探究肺癌组织中长链非编码RNA锌指结构反义转录本1(lncRNA ZFAS1)、hsa-miR-372-3p表达水平及与患者恶性特征及预后的关系。方法选取2015年3月至2016年10月在石家庄市人民医院诊治的82例肺癌患者。RT-qPCR检测肿瘤组织和癌旁组织ZFAS1和miR-372-3p的表达,分析ZFAS1、miR-372-3p水平与肺癌患者恶性特征的关系;Pearson分析肿瘤组织中ZFAS1和miR-372-3p的关系; 用Kaplan-Meier分析ZFAS1、miR-372-3p水平与肺癌患者预后生存期的关系;COX回归分析对肺癌患者预后产生影响的因素。结果与癌旁组织相比,肿瘤组织中ZFAS1、miR-372-3p表达水平均显著升高(P＜0.05)。肿瘤组织ZFAS1、miR-372-3p表达水平与TNM分期、浸润胸膜、是否发生淋巴结转移、肿瘤分化程度有关(P＜0.05)。肿瘤组织中ZFAS1和miR-372-3p表达水平呈显著正相关(r=0.477,P＜0.001)。ZFAS1和miR-372-3p高表达组患者5年内生存率均明显低于低表达组(P＜0.05)。TNM分期、淋巴结转移、ZFAS1、miR-372-3p是肺癌患者发生不良预后的独立危险因素(P＜0.05)。结论肿瘤组织中ZFAS1、miR-372-3p呈异常高表达,其高水平与肺癌患者恶性特征及预后不良有关,是肺癌患者预后不良的独立危险因素。

关键词: 肺癌, 长链非编码RNA, 锌指结构反义转录本1, hsa-miR-372-3p

Abstract: Objective To study the correlation of the expression of long non-coding RNA zinc finger antisense 1 (lncRNA ZFAS1) and hsa-miR-372-3p in lung cancer tissues with the malignant characteristics and prognosis of patients. Methods Eighty-two lung cancer patients hospitalized in Shijiazhuang People's Hospital from March 2015 to October 2016 were selected. The expression of ZFAS1 and miR-372-3p in lung cancer tissues and in adjacent tissues, the potential correlation of the level of ZFAS1 and miR-372-3p with malignant characteristics of patients were analyzed; Pearson method was used to analyze the relationship between ZFAS1 and miR-372-3p in lung cancer tissues; Kaplan-Meier was used to analyze the correlation of the expression of ZFAS1 and miR-372-3p with the prognosis of patients; COX regression was used to analyze the risk factors for poor prognosis of lung cancer patients. Results Compared with the situation of adjacent tissues, the expression of ZFAS1 and miR-372-3p in tumor tissue was significantly higher (P＜0.05). The expression of ZFAS1 and miR-372-3p in tumor tissue was related to TNM staging, lung membrane infiltration, whether lymph node metastasis occurred, and the degree of tumor differentiation (P＜0.05). The expression of ZFAS1 and miR-372-3p content in lung cancer tissues was significantly and positively correlated (r=0.477, P＜0.001). The 5-year survival rate of patients in the ZFAS1 and miR-372-3p high expression group was significantly lower than the low expression group (P＜0.05). TNM staging, lymph node metastasis, ZFAS1, and miR-372-3p were independent risk factors for poor prognosis in patients with lung cancer(P＜0.05). Conclusions ZFAS1 and miR-372-3p are highly expressed in lung cancer tissues and the expression level is related to the malignant characteristics and prognosis of lung cancer patients, so it may be regarded as an independent risk factor to predict poor prognosis of lung cancer patients.

Key words: lung cancer, long non-coding RNA, zinc finger antisense 1, hsa-miR-372-3p

中图分类号:

R734.2

杨晔, 党荣广, 张静, 董超, 宋雪晶. 肺癌组织中lncRNA ZFAS1和hsa-miR-372-3p的表达与患者恶性特征及预后的关系[J]. 基础医学与临床, 2022, 42(12): 1861-1866.

YANG Ye, DANG Rong-guang, ZHANG Jing, DONG Chao, SONG Xue-jing. Correlation of expression of lncRNA ZFAS1 and hsa-miR-372-3p in lung cancer tissues with the malignant characteristics and prognosis of patients[J]. Basic & Clinical Medicine, 2022, 42(12): 1861-1866.

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肺癌组织中lncRNA ZFAS1和hsa-miR-372-3p的表达与患者恶性特征及预后的关系

Correlation of expression of lncRNA ZFAS1 and hsa-miR-372-3p in lung cancer tissues with the malignant characteristics and prognosis of patients

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