Lnc000727促进人骨髓间充质干细胞向成骨细胞分化

doi:10.16352/j.issn.1001-6325.2022.07.1047

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (7): 1047-1052.doi: 10.16352/j.issn.1001-6325.2022.07.1047

Lnc000727促进人骨髓间充质干细胞向成骨细胞分化

蒲超¹, 张珊珊², 吴青霞¹, 罗栩伟¹, 王亮¹, 张波^1*

川北医学院第二临床学院南充市中心医院 1.骨科; 2.神经内科, 四川南充 637000

收稿日期:2021-05-24 修回日期:2021-10-09 出版日期:2022-07-05 发布日期:2022-06-29
通讯作者: * 1138340730@qq.com
基金资助:
四川省教育厅科研计划(17ZB0176);南充市市校合作科研项目(19SXHZ0119)

Lnc000727 promotes differentiation of human bone marrow mesenchymal stem cells into osteoblasts

PU Chao¹, ZHANG Shan-shan², WU Qing-xia¹, LUO Xu-wei¹, WANG Liang¹, ZHANG Bo^1*

1. Department of Orthopedics; 2. Department of Neurology, Nanchong Central Hospital, School of Clinical Medicine, North Sichuan Medical College, Nanchong 637000, China

Received:2021-05-24 Revised:2021-10-09 Online:2022-07-05 Published:2022-06-29
Contact: * 1138340730@qq.com

摘要/Abstract

摘要： 目的探讨lnc000727对人骨髓间充质干细胞(hBM-MSCs)向成骨细胞分化的作用。方法采用实时荧光定量PCR(RT-qPCR)检测lnc000727的表达;用碱性磷酸酶(ALP)法测定成骨细胞分化;通过脂质体转染法过表达或抑制hBM-MSCs中lnc000727的表达;采用RT-qPCR和Western blot检测成骨分化标志物Runt相关转录因子2(RUNX2)、骨钙素(OCN)、骨桥蛋白(OPN) mRNA和蛋白表达;分析Wnt/β-catenin通路关键蛋白质表达。结果在hBM-MSCs成骨细胞分化过程中lnc000727的表达和ALP活性明显上升(P＜0.05)。抑制lnc000727能够下调ALP活性,抑制成骨分化标志物RUNX2、OCN和OPN的表达以及Wnt和β-catenin的表达(P＜0.05);过表达lnc000727能够上调ALP活性,促进成骨分化标志物RUNX2、OCN和OPN的表达以及Wnt和β-catenin的表达(P＜0.05)。结论 Lnc000727促进hBM-MSCs向成骨细胞分化,进而抑制骨质疏松症的发生和发展。

关键词: lnc000727, 成骨细胞分化, Wnt/β-catenin信号通路, 骨质疏松症

Abstract: Objective To explore the effect of lnc000727 on the differentiation of human bone marrow mesenchymal stem cells (hBM-MSCs) into osteoblasts. Methods Real-time fluorescent quantitative PCR (RT-qPCR) was used to detect the expression of lnc000727. The alkaline phosphatase (ALP) method was used to determine the differentiation of osteoblasts. The expression of lnc000727 in hBM-MSCs was over-expressed or inhibited by liposome transfection. The osteogenic differentiation markers Runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), and pontin were detected by RT-qPCR and Western blot. Pontin (OPN) mRNA and protein expression. The expression of key proteins in the Wnt/β-catenin pathway was analyzed. Results During the differentiation of hBM-MSCs osteoblasts, the expression of lnc000727 and ALP activity increased significantly(P＜0.05). Inhibition of lnc000727 could down-regulate ALP activity and inhibit the expression of osteogenic differentiation markers RUNX2, OCN, OPN and the expression of Wnt and β-catenin (P＜0.05); over-expression of lnc000727 could up-regulate ALP activity and promote the expressions of osteogenic differentiation markers RUNX2, OCN and OPN, and the expressions of Wnt and β-catenin (P＜0.05). Conclusions Lnc000727 promotes the differentiation of hBM-MSCs into osteoblasts, thereby inhibits the occurrence and development of osteoporosis.

Key words: lnc000727, osteoblast differentiation, Wnt/β-catenin signaling pathway, osteoporosis

中图分类号:

蒲超, 张珊珊, 吴青霞, 罗栩伟, 王亮, 张波. Lnc000727促进人骨髓间充质干细胞向成骨细胞分化[J]. 基础医学与临床, 2022, 42(7): 1047-1052.

PU Chao, ZHANG Shan-shan, WU Qing-xia, LUO Xu-wei, WANG Liang, ZHANG Bo. Lnc000727 promotes differentiation of human bone marrow mesenchymal stem cells into osteoblasts[J]. Basic & Clinical Medicine, 2022, 42(7): 1047-1052.

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Lnc000727促进人骨髓间充质干细胞向成骨细胞分化

Lnc000727 promotes differentiation of human bone marrow mesenchymal stem cells into osteoblasts

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