基础医学与临床 ›› 2022, Vol. 42 ›› Issue (7): 1053-1059.doi: 10.16352/j.issn.1001-6325.2022.07.1053

• 研究论文 • 上一篇    下一篇

麦冬皂苷B抑制人食管鳞状细胞癌细胞系EC9706增殖和迁移

钟鸣, 于修义*, 郭伟溪, 方正   

  1. 厦门大学附属第一医院 胸外科, 福建 厦门 361003
  • 收稿日期:2021-06-11 修回日期:2021-10-09 出版日期:2022-07-05 发布日期:2022-06-29
  • 通讯作者: * xdfyxiuyiyu@126.com
  • 基金资助:
    福建省自然科学基金(2016J01636);厦门市科学技术项目(3502Z20199168)

Ophiopogonin B inhibits proliferation and migration of human esophageal squamous cell carcinoma cell line EC9706

ZHONG Ming, YU Xiu-yi*, GUO Wei-xi, FANG Zheng   

  1. Department of Thoracic Surgery, the First Affiliated Hospital of Xiamen University, Xiamen 361003, China
  • Received:2021-06-11 Revised:2021-10-09 Online:2022-07-05 Published:2022-06-29
  • Contact: * xdfyxiuyiyu@126.com

摘要: 目的 探讨麦冬皂苷B(OP-B)对食管鳞状细胞癌细胞系(EC9706)增殖、迁移和凋亡的影响,分析其机制是否与调控唐氏综合征细胞黏附分子反义1(DSCAM-AS1)和miR-199a-3p表达有关。方法 将EC9706细胞分为对照组、低、中、高OP-B组、si-NC组、si-DSCAM-AS1组、(pcDNA-DSCAM-AS1+OP-B)组。集落形成实验和CCK-8法检测EC9706增殖。流式细胞测量术、划痕愈合实验分析EC9706细胞凋亡率和迁移能力。RT-qPCR检测DSCAM-AS1和miR-199a-3p表达量。双荧光素酶报告基因实验确定DSCAM-AS1和miR-199a-3p靶向关系。结果 OP-B处理将降低EC9706活力、集落形成数、迁移距离和DSCAM-AS1表达量(P<0.05),增加细胞凋亡率和miR-199a-3p表达量(P<0.05)。DSCAM-AS1与miR-199a-3p存在直接相互作用。干扰DSCAM-AS1表达后,EC9706活力、克隆形成数、迁移距离显著降低(P<0.05),细胞凋亡率显著增加(P<0.05)。过表达DSCAM-AS1可逆转OP-B直接处理对EC9706细胞增殖、凋亡和迁移的影响(P<0.05)。结论 OP-B可抑制食管鳞状细胞癌细胞增殖和迁移,诱导细胞凋亡,其机制可能是通过下调DSCAM-AS1/miR-199a-3p途径实现的。

关键词: 麦冬皂苷B, 食管鳞状细胞癌, DSCAM-AS1, miR-199a-3p, 迁移

Abstract: Objective To explore the effect of ophiopogonin B(OP-B) on the biological behavior of esophageal squamous cell carcinoma cell line(EC9706) and its mechanism related to the regulation of Down syndrome cell adhesion molecule antisense 1 (DSCAM-AS1) and miR-199a-3p expression. Methods EC9706 cells were divided into control group, experiment groups with low, medium and high dosage of OP-B, si-NC group, si-DSCAM-AS1 group and (pcDNA-DSCAM-AS1+OP-B) group. Colony formation and CCK-8 method were used to detect EC9706 proliferation. Flow cytometry and scratch healing test were used to analyze the apoptosis rate and migration of EC9706 cells. RT-qPCR was performed to detect the expression of DSCAM-AS1 and miR-199a-3p. The targeting relationship between DSCAM-AS1 and miR-199a-3p was verified using dual luciferase reporter gene experiment. Results OP-B reduced EC9706 viability, colony formation counting, migration distance and DSCAM-AS1 expression (P<0.05), increased cell apoptosis rate and miR-199a-3p expression (P<0.05). DSCAM-AS1 interacted directly with miR-199a-3p. After interference to DSCAM-AS1 expression, the cell viability, colony formation and migration distance of EC9706 cells were significantly reduced(P<0.05), while apoptosis rates were significantly increased(P<0.05). Over-expression of DSCAM-AS1 antagonized the effects of OP-B on the proliferation, apoptosis and migration of EC9706 cells(P<0.05). Conclusions OP-B inhibits proliferation and migration of esophageal squamous cell carcinoma cells and induces cell apoptosis. The mechanism is potentially explained by down-regulating DSCAM-AS1/miR-199a-3p pathway.

Key words: ophiopogonin B, esophageal squamous cell carcinoma, DSCAM-AS1, miR-199a-3p, migration

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