沉默lnc-SLC2A12-10:1抑制人胃癌细胞系AGS增殖、迁移和侵袭

doi:10.16352/j.issn.1001-6325.2022.07.1071

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (7): 1071-1076.doi: 10.16352/j.issn.1001-6325.2022.07.1071

沉默lnc-SLC2A12-10:1抑制人胃癌细胞系AGS增殖、迁移和侵袭

刘磊¹, 李世宝², 张好良^2*

1.徐州医科大学生理学教研室,江苏徐州 221004;
2.徐州医科大学附属医院检验科,江苏徐州 221002

收稿日期:2021-03-25 修回日期:2021-10-15 出版日期:2022-07-05 发布日期:2022-06-29
通讯作者: * haoliang_z@sina.com
基金资助:
江苏省十三五“科教强卫”青年医学人才项目(QNRC2016781);徐州医科大学附属医院院级科研项目(2020KC017)

Lnc-SLC2A12-10:1 silencing inhibits the proliferation, migration and invasion of gastric cancer cell line AGS

LIU Lei¹, LI Shi-bao², ZHANG Hao-liang^2*

1. Department of Physiology, Xuzhou Medical University, Xuzhou 221004;
2. Department of Laboratory Medicine, the Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, China

Received:2021-03-25 Revised:2021-10-15 Online:2022-07-05 Published:2022-06-29
Contact: * haoliang_z@sina.com

摘要/Abstract

摘要： 目的探讨长链非编码RNA lnc-SLC2A12-10:1沉默对人胃癌细胞系AGS增殖、迁移和侵袭的影响。方法用基因干扰技术构建lnc-SLC2A12-10:1沉默的AGS细胞株。 RT-qPCR验证lnc-SLC2A12-10:1的干扰效率;CCK-8法检测AGS细胞增殖;划痕和Transwell小室法检测AGS细胞迁移和侵袭。软件预测lnc-SLC2A12-10:1靶向微小RNA(miRNA)并RT-qPCR验证。结果 Lnc-SLC2A12-10:1 shRNA能有效干扰lnc-SLC2A12-10:1的表达,lnc-SLC2A12-10:1沉默可抑制胃癌细胞增殖(P＜0.05)。沉默lnc-SLC2A12-10:1还抑制AGS细胞的迁移和侵袭(P＜0.05)。经验证,lnc-SLC2A12-10:1靶向的miRNA可能为hsa-miR-105-5p和hsa-miR-150-3p。结论沉默lnc-SLC2A12-10:1可显著抑制AGS细胞增殖、迁移和侵袭能力。

关键词: 长链非编码RNA, 胃癌, 增殖, 迁移

Abstract: Objective To investigate the effect of long non-coding RNA(lncRNA) lnc-SLC2A12-10:1 silencing on the proliferation, migration and invasion of gastric cancer cell line AGS. Methods Gene interference technology was used to construct the lnc-SLC2A12-10:1 silenced gastric cancer cell strain AGS, and RT-qPCR was used to verify the interference efficiency by lnc-SLC2A12-10:1 silencing. The proliferation of AGS cell was examined by CCK-8 method. The cell migration and invasion were evaluated by wound healing and Transwell assay. Bioinformatics prediction software was applied to predict lnc-SLC2A12-10:1 targeting miRNA and to verify them with RT-qPCR. Results Lnc-SLC2A12-10:1 shRNA effectively silenced expression of lnc-SLC2A12-10:1, and lnc-SLC2A12-10:1 silencing inhibited the proliferation of gastric cancer cells (P＜0.05). Lnc-SLC2A12-10:1 silencing also inhibited the migration and invasion of AGS cells (P＜0.05). It had been demonstrated that lnc-SLC2A12-10:1 targeting miRNAs might be hsa-miR-105-5p and hsa-miR-150-3p. Conclusions Lnc-SLC2A12-10:1 silencing can significantly inhibit the proliferation, migration and invasion of AGS cells.

Key words: long noncoding RNA, gastric cancer, proliferation, migration

中图分类号:

刘磊, 李世宝, 张好良. 沉默lnc-SLC2A12-10:1抑制人胃癌细胞系AGS增殖、迁移和侵袭[J]. 基础医学与临床, 2022, 42(7): 1071-1076.

LIU Lei, LI Shi-bao, ZHANG Hao-liang. Lnc-SLC2A12-10:1 silencing inhibits the proliferation, migration and invasion of gastric cancer cell line AGS[J]. Basic & Clinical Medicine, 2022, 42(7): 1071-1076.

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沉默lnc-SLC2A12-10:1抑制人胃癌细胞系AGS增殖、迁移和侵袭

Lnc-SLC2A12-10:1 silencing inhibits the proliferation, migration and invasion of gastric cancer cell line AGS

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