高脂饲养C57BL/6J小鼠肾周脂肪组织炎性反应的评估

基础医学与临床 ›› 2021, Vol. 41 ›› Issue (9): 1318-1322.

高脂饲养C57BL/6J小鼠肾周脂肪组织炎性反应的评估

杨永¹, 李梓玉¹, 杨伟¹, 刘勇^1,2*

1.西南医科大学附属医院病理科, 四川泸州 646000;
2.核医学与分子影像四川省重点实验室,四川泸州 646000

收稿日期:2020-12-25 修回日期:2021-05-12 出版日期:2021-09-05 发布日期:2021-09-02
通讯作者: *254236278@qq.com
基金资助:
西南医科大学附属医院博士科研启动基金(20012);西南医科大学附属医院科研项目(17128);核医学与分子影像四川省重点实验室2020年开放课题(2020-206)

Evaluation of inflammation of perirenal adipose tissue in C57BL/6J mice with high-fat diet

YANG Yong¹, LI Zi-yu¹, YANG Wei¹, LIU Yong^1,2*

1. Department of Pathology, the Affiliated Hospital of Southwest Medical University, Luzhou 646000;
2. Nuclear Medicine and Molecular Imaging Key Laboratory of Sichuan Province, Luzhou 646000, China

Received:2020-12-25 Revised:2021-05-12 Online:2021-09-05 Published:2021-09-02
Contact: *254236278@qq.com

摘要/Abstract

摘要： 目的评估高脂饲养C57BL/6J小鼠肾周脂肪组织炎性反应。方法将小鼠随机分为对照组(control组,n=6)和高脂饲养组(HFD组,n=6)。用RT-qPCR检测肾周脂肪组织中TNF-α、CD11c、IL-1β、IL-10、TGF-β1、CD206等mRNA的表达;免疫组织化学法检测肾周脂肪组织及肾实质F4/80、CD68、LCA的水平。结果与对照组比较,HFD组小鼠肾周脂肪组织TNF-α、IL-1β等mRNA相对含量升高(P＜0.05);HFD组的肾周脂肪巨噬细胞标志物F4/80,CD68及炎细胞广谱标志物LCA的表达均呈高表达(P＜0.05)。结论高脂饲养的C57BL/6J小鼠肾周脂肪组织确实存在明显的炎性反应。

关键词: 脂肪组织, 高脂饲养, 炎性反应, 肾, 巨噬细胞

Abstract: Objective To evaluate the inflammation of perirenal adipose tissue induced by high-fat diet in C57BL/6J mice. Methods Mice were randomly divided into normal diet group (control group, n=6) and high-fat diet group (HFD group, n=6). The mRNA expression of TNF-α, CD11c, IL-1β, IL-10, TGF-β1, CD206 in perirenal adipocytes were detected by RT-qPCR. The expressions of F4/80, CD68 and LCA in perirenal adipose tissue and renal parenchyma were detected by immunohistochemistry method. Results Compared with the control group, the mRNA expression by TNF-α and IL-1β in perirenal adipose tissue of mice in HFD group was increased(P＜0.05); The expression of F4/80, CD68 and LCA in perirenal fat increased in HFD group(P＜0.05). Conclusions High-fat diet induces inflammation in perirenal adipose tissue of C57BL/6J mice.

Key words: adipose tissue, high-fat diet, inflammation, kidney, macrophages

中图分类号:

R364.5

杨永, 李梓玉, 杨伟, 刘勇. 高脂饲养C57BL/6J小鼠肾周脂肪组织炎性反应的评估[J]. 基础医学与临床, 2021, 41(9): 1318-1322.

YANG Yong, LI Zi-yu, YANG Wei, LIU Yong. Evaluation of inflammation of perirenal adipose tissue in C57BL/6J mice with high-fat diet[J]. Basic & Clinical Medicine, 2021, 41(9): 1318-1322.

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