miR-760对胃癌细胞系MGC-803增殖、迁移和侵袭的影响

基础医学与临床 ›› 2016, Vol. 36 ›› Issue (2): 207-210.

miR-760对胃癌细胞系MGC-803增殖、迁移和侵袭的影响

李峰¹,王芳²,梁爱华¹

1. 山西大学
2. 中国医学科学院基础医学研究所北京协和医学院基础学院

收稿日期:2015-11-09 修回日期:2015-12-18 出版日期:2016-02-05 发布日期:2016-01-21
通讯作者: 梁爱华 E-mail:1029090213@qq.com
基金资助:
国家自然科学基金

Effects of miR-760 on cell proliferation, migration and invasion of gastric cancer cell lines MGC-803

¹,Fang WANGAi-Hua Liang

Received:2015-11-09 Revised:2015-12-18 Online:2016-02-05 Published:2016-01-21
Contact: Ai-Hua Liang E-mail:1029090213@qq.com

摘要/Abstract

摘要： 目的探讨miR-760对胃癌细胞系MGC-803增殖、迁移和侵袭的影响。方法Real-time PCR 分析50例胃癌组织（C）及其癌旁（N）中miR-760的表达水平；用pcDNA3.1载体构建过表达miR-760的重组质粒（pcDNA-miR-760），实现miR-760在MGC-803细胞中的过表达；分别用CCK-8法、Transwell和划痕实验检测细胞增殖、侵袭和迁移能力。结果与癌旁对照组相比，36例（72%）胃癌组织中出现miR-760的表达下调；过表达miR-760能显著抑制MGC-803细胞的迁移和侵袭能力（p<0.05），但对其增殖影响不大。结论 miR-760的表达下调可能与胃癌的进展有关，过表达miR-760可以抑制胃癌MGC-803细胞的迁移和侵袭。

关键词: 关键词：miR-760, 胃癌, 增殖, 迁移, 侵袭

Abstract: Objective To investigate the effects of miR-760 on cell proliferation, migration and invasion of gastric cancer cell line MGC-803. Methods The level of miR-760 expression was detected by real-time RT-PCR in 50 paired gastric cancer tissues and their adjacent normal tissues. miR-760 overexpression was achieved by transfection of construct pcDNA-miR-760 into MGC-803 cells. The proliferation, migration and invasion of MGC-803 cells were detected by the CCK-8, Transwell and scratch wound assay, respectively. Results miR-760 was decreased in 36 cases (72%) of gastric cancer tissues compared to their control. Furthermore, overexpression of miR-760 effectively inhibited the migration and invasion of MGC-803 cells（p<0.05）, but had no effects on proliferation. Conclusion Down-regulation of miR-760 has a correlation with the progression of gastric cancer. Up-regulation of miR-760 can inhibit the invasion and migration of MGC-803 cells.

Key words: Key words：miR-760, gastric cancer, proliferation, migration, invasion

中图分类号:

Q527

李峰王芳梁爱华. miR-760对胃癌细胞系MGC-803增殖、迁移和侵袭的影响[J]. 基础医学与临床, 2016, 36(2): 207-210.

Fang WANG Ai-Hua Liang. Effects of miR-760 on cell proliferation, migration and invasion of gastric cancer cell lines MGC-803[J]. Basic & Clinical Medicine, 2016, 36(2): 207-210.

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