关键词: 肺腺癌, 淋巴结转移, 转录组测序, 差异表达基因

Abstract: Objective: Using the transcriptome sequencing and bioimformatics methods to identify the mediastinal lymph node metastasis associated genes of lung adenocarcinoma. Methods: Using the transcriptome sequencing method, we detected 10 cases of lung adenocarcinoma samples with EGFR sensitive mutation. Comparing the two groups of the transcriptome expression profiles, Differentially expressed genes (DEGs) were analyzed, bioimformatics methods were used to analyze the role of these genes. q-PCR detected the expression of the upregurated genes in PC-9 cell lines (EGFR sensitive mutation cell lines) . Results: There were 68 DEGS up-regulated and 101 genes down-regulated in the lung adenocarcinoma samples. q-PCR showed that the aboundance of 7 genes(ZNF572,BRPF3,MMACHC and so on.) in upregurated genes was low, nine genes(SMOC1,A1BG,BARX1 and so on.) was moderate, and others was high. Conclusion: TFF3, DUSP6 and other DEGs may be involved in the process of mediastinal lymph node metastasis in lung adenocarcinoma.

Key words: lung adenocarcinoma, lymph node metastasis, transcriptome sequencing, differentially expressed genes