老年血液透析患者血清miR-21与T细胞相关

doi:10.16352/j.issn.1001-6325.2022.08.1188

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (8): 1188-1193.doi: 10.16352/j.issn.1001-6325.2022.08.1188

老年血液透析患者血清miR-21与T细胞相关

王珊, 杨华昱, 孙颖, 马清^*

首都医科大学附属北京友谊医院老年医学科, 北京 100050

收稿日期:2022-06-06 修回日期:2022-06-13 出版日期:2022-08-05 发布日期:2022-08-01
通讯作者: ^*maqing3@163.com
基金资助:
国家自然科学基金(81601210);北京市医院管理局“青苗”计划(QML20170103)

Correlation between serum miR-21 expression and T cells in elderly dialysis patients

WANG Shan, YANG Hua-yu, SUN Ying, MA Qing^*

Department of Geriatrics, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China

Received:2022-06-06 Revised:2022-06-13 Online:2022-08-05 Published:2022-08-01
Contact: ^*maqing3@163.com

摘要/Abstract

摘要： 目的探讨老年透析患者miR-21与外周血T淋巴细胞亚群之间的相关性。方法将2020年1月至2021年1月北京友谊医院老年医学科收治的老年维持性血液透析患者28例纳入观察组,47例老年健康体检者纳入对照组,检测对照组入组时及观察组透析前外周血T淋巴细胞亚群及miR-21、miR-23a、miR-150、miR-100、miR-128、miR-211表达水平,以及透析前后miR-21与CD4⁺、Treg细胞百分率的相关性。结果与对照组相比,观察组透析前收缩压、尿素氮、中性粒细胞百分比、白介素-6水平明显增高,血红蛋白、谷丙转氨酶、白蛋白、估算的肾小球滤过率明显偏低(P＜0.001),CD4⁺和Treg细胞百分率均显著增高(P＜0.001),miR-21-5p相对表达量有所增高(P＜0.05)。透析后观察组估算的肾小球滤过率、尿素氮、血磷、血钾水平明显下降(P＜0.001),且透析后miR-21相对表达量和Treg细胞的比率均增高(P＜0.05), Pearson相关分析显示观察组在透析前后的miR-21相对表达量和Treg细胞比率呈正相关(P＜0.05)。结论老年透析患者存在微炎性反应状态,透析前后血清miR-21表达量与Treg细胞比率呈正相关。

关键词: 老年, 血液透析, T淋巴细胞, 微炎性反应, miR-21

Abstract: Objective To investigate the correlation between miR-21 and peripheral blood lymphocyte subsets in elderly dialysis patients. Methods Twenty-eight elderly maintenance hemodialysis patients were enrolled as the observation group from the Department of Geriatrics of Beijing Friendship Hospital from January 2020 to January 2021. Forty-seven elderly healthy check-up people were included in the control group. The percentage of T lymphocyte subsets and the expression levels of miR-21, miR-23a, miR-150, miR-100; miR-128 and miR-211 were examined in control group and observation group. The percentage of CD4⁺ and Treg cells and the expression levels of miR-21 were detected before and after dialysis. Results The levels of systolic blood pressure, urea nitrogen, neutrophile percentage and interleukin-6 in patients with the observation group were significantly higher than that of the control group (P＜0.001). The levels of estimated glomerular filtration rate, hemoglobin, glutamic-pyruvate transaminase, albumin were significantly lower in the observation group(P＜0.001), and the percentages of CD4⁺ and Treg cells were significantly increased(P＜0.001). The relative expression of miR-21-5p was increased in the observation group(P＜0.05). Pearson correlation analysis showed that the relative expression of miR-21 was positively correlated with the ratio of Treg cells in the observation group before and after dialysis (P＜0.05). Conclusions There are micro-inflammation in elderly patients undergoing dialysis and the expression level of miR-21 in serum is positively correlated with the ratio of Treg cells before and after dialysis.

Key words: elderly, hemodialysis, T lymphocyte, micro-inflammation, miR-21

中图分类号:

R459.5

王珊, 杨华昱, 孙颖, 马清. 老年血液透析患者血清miR-21与T细胞相关[J]. 基础医学与临床, 2022, 42(8): 1188-1193.

WANG Shan, YANG Hua-yu, SUN Ying, MA Qing. Correlation between serum miR-21 expression and T cells in elderly dialysis patients[J]. Basic & Clinical Medicine, 2022, 42(8): 1188-1193.

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老年血液透析患者血清miR-21与T细胞相关

Correlation between serum miR-21 expression and T cells in elderly dialysis patients

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