下调lncRNA RHPN1-AS1抑制人膀胱癌细胞系T24增殖和迁移

doi:10.16352/j.issn.1001-6325.2022.06.004

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (6): 940-944.doi: 10.16352/j.issn.1001-6325.2022.06.004

下调lncRNA RHPN1-AS1抑制人膀胱癌细胞系T24增殖和迁移

白冰, 张海芳^*, 杨庆良, 秦悦, 周晨龙, 宋歌, 王颖

安阳市人民医院泌尿外科, 河南安阳 455000

收稿日期:2020-10-12 修回日期:2021-01-22 出版日期:2022-06-05 发布日期:2022-06-02
通讯作者: * 398713172@qq.com
基金资助:
河南省医学科技攻关计划联合共建项目(LHGJ20191265)

Down-regulation of lncRNA RHPN1-AS1 inhibits proliferation and migration of human bladder cancer cell line T24

BAI Bing, ZHANG Hai-fang^*, YANG Qing-liang, QIN Yue, ZHOU Chen-long, SONG Ge, WANG Ying

Department of Urology, Anyang People's Hospital, Anyang 455000, China

Received:2020-10-12 Revised:2021-01-22 Online:2022-06-05 Published:2022-06-02
Contact: * 398713172@qq.com

摘要/Abstract

摘要： 目的探讨干扰lncRNA RHPN1-AS1对膀胱癌细胞系T24生物学行为的影响及作用机制。方法用RT-qPCR检测膀胱癌组织与癌旁组织中RHPN1-AS1、miR-149-3p的表达;分别将si-NC、si-RHPN1-AS1、si-RHPN1-AS1与anti-miR-NC、si-RHPN1-AS1与miR-149-3p inhibitor转染至T24细胞;用RT-qPCR检测细胞中RHPN1-AS1、miR-149-3p的表达水平;MTT法、平板集落形成实验、划痕实验、流式细胞测量术分别检测细胞增殖、迁移能力及细胞凋亡率;双荧光素酶报告基因实验检测RHPN1-AS1与miR-149-3p的靶向关系;Western blot检测B淋巴细胞瘤-2相关蛋白(Bax)及B淋巴细胞瘤-2(Bcl-2)蛋白表达。结果膀胱癌组织中RHPN1-AS1的表达水平高于癌旁组织(P＜0.05),miR-149-3p的表达水平低于癌旁组织(P＜0.05);与si-NC组比较,转染si-RHPN1-AS1可明显降低细胞增殖能力、划痕愈合率及Bcl-2蛋白水平(P＜0.05),减少集落形成数(P＜0.05),提高凋亡率及Bax蛋白水平(P＜0.05);miR-149-3p是RHPN1-AS1的靶基因;与si-RHPN1-AS1+anti-miR-NC组比较,转染si-RHPN1-AS1与miR-149-3p inhibitor可明显提高细胞增殖能力、划痕愈合率及Bcl-2蛋白水平(P＜0.05),增加集落形成数(P＜0.05),降低凋亡率及Bax蛋白水平(P＜0.05)。结论干扰RHPN1-AS1表达可通过上调miR-149-3p从而抑制膀胱癌细胞增殖、迁移及促进细胞凋亡。

关键词: lncRNARHPN1-AS1, miR-149-3p, 膀胱癌, 增殖, 迁移

Abstract: Objective To explore the effect of interfering with lncRNA RHPN1-AS1 on the biological behavior of bladder cancer cell T24 and its mechanism. Methods RT-qPCR method was used to detect the expression of RHPN1-AS1 and miR-149-3p by bladder cancer tissues and adjacent tissues.si-NC,si-RHPN1-AS1, si-RHPN1-AS1,anti-miR-NC, si-RHPN1-AS1 and miR-149-3p inhibitor were transfected into bladder cancer cell T24. The expression of RHPN1-AS1 and miR-149-3p in T24 cells were examined by RT-qPCR.MTT, plate colony formation experiment, scratch experiment, flow cytometry were used to detect cell proliferation, migration ability and cell apoptosis rate respectively. The dual luciferase reporter experiment was used to detect the targeting relationship between RHPN1-AS1 and miR-149-3p. Bax and Bcl-2 protein expression was assessed using Western blot. Results Compared with adjacent tissues, the expression of RHPN1-AS1 in bladder cancer tissue was increased(P＜0.05) and the expression of miR-149-3p was decreased (P＜0.05). Compared with the si-NC group, transfection of si-RHPN1-AS1 significantly reduced cell proliferation, scratch healing rate and the protein level of Bcl-2(P＜0.05), reduced the number of clones (P＜0.05)and increased apoptosis rate and the protein level of Bax(P＜0.05). Compared with the si-RHPN1-AS1+anti-miR-NC group, transfection of si-RHPN1-AS1 and miR-149-3p inhibitor significantly increased cell proliferation, scratch healing rate and the protein level of Bcl-2 (P＜0.05) increased the number of clones formed (P＜0.05), reduced the apoptosis rate and the protein level of Bax (P＜0.05). Conclusions Interfering with the expression of RHPN1-AS1 may inhibit the proliferation and migration of bladder cancer cells and may promote cell apoptosis by up-regulating miR-149-3p.

Key words: lncRNA RHPN1-AS1, miR-149-3p, bladder cancer, proliferation, migration

中图分类号:

R737.1

白冰, 张海芳, 杨庆良, 秦悦, 周晨龙, 宋歌, 王颖. 下调lncRNA RHPN1-AS1抑制人膀胱癌细胞系T24增殖和迁移[J]. 基础医学与临床, 2022, 42(6): 940-944.

BAI Bing, ZHANG Hai-fang, YANG Qing-liang, QIN Yue, ZHOU Chen-long, SONG Ge, WANG Ying. Down-regulation of lncRNA RHPN1-AS1 inhibits proliferation and migration of human bladder cancer cell line T24[J]. Basic & Clinical Medicine, 2022, 42(6): 940-944.

参考文献

[1] 黄一珂, 刁思军, 梁平, 等. 靶向沉默膀胱癌T24细胞系VEGF对树突状细胞分化成熟及免疫功能的影响[J]. 基础医学与临床, 2018, 38: 57-62.
[2] Luo H, Xu C, Le W, et al. lncRNA CASC11 promotes cancer cell proliferation in bladder cancer through miRNA-150[J]. J Cell Biochem, 2019, 120: 13487-13493.
[3] Zhuang C, Ma Q, Zhuang C, et al. LncRNA GClnc1 promotes proliferation and invasion of bladder cancer through activation of MYC[J]. FASEB J, 2019, 33: 11045-11059.
[4] Li HJ, Sun XM, Li ZK, et al. LncRNA UCA1 promotes mitochondrial function of bladder cancer via the miR-195/ARL2 signaling pathway[J]. Cell Physiol Biochem, 2017, 43: 2548-2561.
[5] Duan H, Li X, Chen Y, et al. LncRNA RHPN1-AS1 promoted cell proliferation, invasion and migration in cervical cancer via the modulation of miR-299-3p/FGF2 axis[J]. Life Sci, 2019, 239: 116856-116866.
[6] Qi H, Xiao Z, Wang Y. Long non-coding RNA LINC00665 gastric cancer tumorigenesis by regulation miR-149-3p/RNF2 axis[J]. Onco Targets Ther, 2019, 12: 6981-6990.
[7] Zhao L, Liu T, Zhang X, et al. lncRNA RHPN1-AS1 promotes ovarian cancer growth and invasiveness through inhibiting miR-1299[J]. Onco Targets Ther, 2020, 13: 5337-5344.
[8] Zheng W, Li H, Zhang H, et al. Long noncoding RNA RHPN1-AS1 promotes colorectal cancer progression via targeting miR-7-5p/OGT axis[J]. Cancer Cell Int, 2020, 20: 54-64.
[9] Liu G, Zhao X, Zhou J, et al. Long non-coding RNA MEG3 suppresses the development of bladder urothelial carcinoma by regulating miR-96 and TPM1[J]. Cancer Biol Ther, 2018, 19: 1039-1056.
[10] Chen D, Zhang M, Ruan J, et al. The long non-coding RNA HOXA11-AS promotes epithelial mesenchymal transition by sponging miR-149-3p in Colorectal Cancer[J]. J Cancer, 2020, 11: 6050-6058.
[11] Liang Y, Hou L, Li L, et al. Dichloroacetate restores colorectal cancer chemosensitivity through the p53/miR-149-3p/PDK2-mediated glucose metabolic pathway[J]. Oncogene, 2020, 39: 469-485.
[12] Liu Y, Yang C, Zhao Y, et al. Overexpressed methyltransferase-like 1 (METTL1) increased chemosensitivity of colon cancer cells to cisplatin by regulating miR-149-3p/S100A4/p53 axis[J]. Aging (Albany NY), 2019, 11: 12328-12344.

[1]	刘磊, 李世宝, 张好良. 沉默lnc-SLC2A12-10:1抑制人胃癌细胞系AGS增殖、迁移和侵袭[J]. 基础医学与临床, 2022, 42(7): 1071-1076.
[2]	钟鸣, 于修义, 郭伟溪, 方正. 麦冬皂苷B抑制人食管鳞状细胞癌细胞系EC9706增殖和迁移[J]. 基础医学与临床, 2022, 42(7): 1053-1059.
[3]	康有力, 赵丹, 杜文静, 李莉. SIRT5通过调控ALDH5A1的琥珀酰化促进人乳腺癌细胞的增殖[J]. 基础医学与临床, 2022, 42(7): 1099-1107.
[4]	杜雪梅, 张运梅, 周文婷, 吕宏. miR-655-3p靶向KIF20A抑制人肺腺癌细胞系A549迁移及侵袭[J]. 基础医学与临床, 2022, 42(4): 599-606.
[5]	孙全鹏, 樊超, 展德玺, 范怡明, 孙伟峰. 罗哌卡因抑制人肝癌细胞系Hep3B增殖、迁移和侵袭[J]. 基础医学与临床, 2022, 42(3): 448-453.
[6]	张健, 张吉炯. LncRNA UNC5B-AS1靶向miR-339-5p调控人肺腺癌细胞系A549增殖和凋亡[J]. 基础医学与临床, 2022, 42(3): 454-460.
[7]	杨芒庄, 杨旭东, 王晓龙. Circ-MALAT1靶向miR-101抑制膀胱癌细胞系SW780侵袭和迁移[J]. 基础医学与临床, 2022, 42(3): 472-478.
[8]	刘新兵, 张美红. 钩吻碱通过下调circ_001680抑制类风湿关节炎滑膜成纤维细胞增殖和侵袭[J]. 基础医学与临床, 2022, 42(3): 429-435.
[9]	赵霞, 蔡庆春, 丁瑞敏, 张倩. 华蟾素抑制人鼻咽癌细胞系CNE2和HONE1增殖[J]. 基础医学与临床, 2022, 42(3): 436-440.
[10]	陈茜, 刘宪, 崔南, 翟阳. 敲除EZH2抑制人宫颈癌细胞系迁移和侵袭[J]. 基础医学与临床, 2022, 42(3): 395-400.
[11]	张春利, 齐结华, 宦宇, 吴守乐, 蔡徐山. 干扰ADAM17抑制人宫颈癌细胞系HeLa细胞增殖[J]. 基础医学与临床, 2022, 42(2): 255-259.
[12]	王布, 袁胜芳, 张长洪, 苑程, 黄攀登, 张志华, 赵建清. eEF2K沉默联合丹参酮ⅡA磺酸钠协同抑制人肺腺癌细胞系A549增殖[J]. 基础医学与临床, 2022, 42(1): 106-113.
[13]	李勇晓, 孙燕, 张瑞生. 丙泊酚抑制人子宫内膜癌细胞系RL95-2增殖[J]. 基础医学与临床, 2022, 42(1): 114-119.
[14]	王灵霞, 严玉兰, 袁海涛, 蔡烨伟, 刘德慧, 谷文露, 曹碧月. CircRNA_23113抑制肺腺癌细胞的增殖和迁移[J]. 基础医学与临床, 2022, 42(1): 82-88.
[15]	史铁伟, 李天柱, 周静, 娜日苏, 孙琪, 许丽艳, 白春英. 5-脂氧合酶激活蛋白抑制剂MK886对人食管癌细胞系增殖和凋亡的影响[J]. 基础医学与临床, 2022, 42(1): 56-61.

下调lncRNA RHPN1-AS1抑制人膀胱癌细胞系T24增殖和迁移

Down-regulation of lncRNA RHPN1-AS1 inhibits proliferation and migration of human bladder cancer cell line T24

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价