基础医学与临床 ›› 2022, Vol. 42 ›› Issue (1): 82-88.

• 研究论文 • 上一篇    下一篇

CircRNA_23113抑制肺腺癌细胞的增殖和迁移

王灵霞1,严玉兰2,袁海涛1,蔡烨伟1,刘德慧1,谷文露1,曹碧月1   

  1. 1. 江苏大学医学院
    2. 江苏大学附属人民医院
  • 收稿日期:2020-12-14 修回日期:2021-05-07 出版日期:2022-01-05 发布日期:2022-01-05
  • 通讯作者: 严玉兰 E-mail:ylyan2005@163.com
  • 基金资助:
    江苏省自然科学基金;镇江市重点研发计划项

CircRNA_23113 inhibits the proliferation and migration of lung adenocarcinoma cells

  • Received:2020-12-14 Revised:2021-05-07 Online:2022-01-05 Published:2022-01-05
  • Supported by:
    Natural Science Foundation of Jiangsu Province;Science and Technology Planning Social Development Project of Zhenjiang City

摘要: 目的:探究circRNA_23113在肺腺癌中的表达及对肺腺癌细胞增殖和迁移的影响。方法:通过高通量测序技术测序5例临床肺腺癌、癌旁组织标本,筛选出差异性表达的环状RNA circRNA_23113。实时荧光定量PCR检测肺腺癌组织、血清和细胞中circRNA_23113的表达;构建circRNA_23113的过表达质粒,用CCK-8法、克隆形成实验、细胞划痕和transwell小室法分析其对细胞增殖和迁移的影响;用Western blot 检测β-catenin、cyclin D1以及 c-myc蛋白水平的表达。结果:CircRNA_23113在肺腺癌患者组织、血清和细胞中显著低表达(P<0.05)。过表达circRNA_23113后抑制A549和H1299细胞增殖和迁移(P<0.05);circRNA_23113抑制β-catenin、cyclin D1以及 c-myc 表达(P<0.05)。结论:circRNA_23113在肺腺癌中低表达,且过表达能抑制肺腺癌细胞的增殖和迁移。

关键词: circRNA_23113, 肺腺癌, 增殖和迁移

Abstract: Objective: To explore the expression of circRNA_23113 in lung adenocarcinoma and its influence on the proliferation and migration of lung adenocarcinoma cells. Methods: Screening 5 cases of lung adenocarcinoma and adjacent tissues by high-throughput sequencing,from which screened out the differentially expressed circular RNA circRNA_23113. Real-time fluorescence quantitative PCR was used to detect the expression of circRNA_23113 in lung adenocarcinoma tissues, serum and cells; the overexpression plasmid of circRNA_23113 was constructed, and its effect on cell proliferation and migration was analyzed by CCK-8 method, clone formation experiment, cell scratching and transwell chamber method; Westernblot was used to detect the expression of β-catenin, cyclinD1 and c-myc protein levels. Results: CircRNA_23113 was significantly lower expressed in tissues, serum and cells of patients with lung adenocarcinoma (P<0.05). Overexpression of circRNA_23113 inhibited the proliferation and migration of A549 and H1299 cells(P<0.05); circRNA_23113 inhibited the expression of β-catenin, cyclinD1 and c-myc(P<0.05). Conclusion:circRNA_23113 is poorly expressed in lung adenocarcinoma, and overexpression can inhibit the proliferation and migration of lung adenocarcinoma cells.

Key words: circRNA_23113, lung adenocarcinoma, proliferation and migration