全转录组测序分析精子发生中RNA结合蛋白质的动态表达

基础医学与临床 ›› 2021, Vol. 41 ›› Issue (6): 825-830.

全转录组测序分析精子发生中RNA结合蛋白质的动态表达

李凯, 邙新雨, 邹定峰, 李梦真, 缪时英, 王琳芳, 宋伟^*

中国医学科学院基础医学研究所北京协和医学院基础学院生物化学与分子生物学系医学分子生物学国家重点实验室, 北京 100005

收稿日期:2021-03-29 修回日期:2021-04-16 出版日期:2021-06-05 发布日期:2021-05-31
通讯作者: ^*songwei@ibms.pumc.edu.cn
基金资助:
国家自然科学基金(31970794,32000586)

Dynamic expression of RNA-binding proteins in spermatogenesis based on RNA-seq

LI Kai, MANG Xin-yu, ZOU Ding-feng, LI Meng-zhen, MIAO Shi-ying, WANG Lin-fang, SONG Wei^*

State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing 100005, China

Received:2021-03-29 Revised:2021-04-16 Online:2021-06-05 Published:2021-05-31
Contact: ^*songwei@ibms.pumc.edu.cn

摘要/Abstract

摘要： 目的系统解析RNA结合蛋白质(RBPs)在小鼠精子发生中的动态表达全貌、阶段特异性及协同表达模式,并预测其潜在调控作用。方法整合6种类型生精细胞的全转录组测序数据,分析精子发生全程差异表达的RBPs;利用时间序列分析软件(STEM)分析差异表达RBPs动态表达模式;利用加权基因共表达网络分析(WGCNA)鉴定精子发生中协同表达的RBPs;通过ClusterProfiler工具分别对差异表达以及协同表达的RBPs进行GO功能富集分析。结果精子发生中共鉴定519个阶段特异表达的RBPs,并具有7种动态表达模式,其中减数分裂时期的RBPs占比最高;GO分析显示RBPs主要参与mRNA选择性剪接、加工或翻译过程;WGCNA分析获得246个共表达RBPs,其中减数分裂时期共表达RBPs占比最高。结论 RBPs在精子发生中呈现阶段特异性,并且以协同表达模式发挥调控作用。其在精子发生早期阶段参与RNA加工或剪接等过程,而在后期阶段参与核糖体组装或RNA翻译等过程。

关键词: RNA结合蛋白质, 转录组, 共表达, 精子发生

Abstract: Objective To systematically characterize the dynamic expression pattern, stage specificity and co-expression pattern of RNA-binding protein (RBPs) and to predict their potential regulatory role in mouse spermatogenesis. Methods The whole transcriptome sequencing data of six spermatogenic cells types were integrated for analyzing the differentially expressed RBPs during spermatogenesis;STEM was used to analyze the dynamic expression pattern of RBPs; WGCNA was used to identify the RBPs co-expressed pattern; The differentially expressed and co-expressed RBPs were analyzed by the ClusterProfiler tool for GO function enrichment analysis. Results A total of 519 stage-specific RBPs were identified during spermatogenesis, and there were 7 dynamic expression patterns, of which RBPs at the meiotic stage accounted for the highest proportion; GO enrichment analysis showed that RBPs were mainly involved in the selective splicing, processing or translation of mRNA; WGCNA analysis showed that 246 RBPs were co-expressed, among which RBPs at the meiotic stage accounted for the highest proportion. Conclusions RBPs exhibit stage specificity and play a regulatory role in spermatogenesis with a coordinated expression mode. It functions mainly in the process of RNA processing or splicing in the early stage of spermatogenesis, and is have significant impact on the process of ribosome assembly or RNA translation in the later stage.

Key words: RNA-binding proteins, transcriptome, co-expression, spermatogenesis

中图分类号:

李凯, 邙新雨, 邹定峰, 李梦真, 缪时英, 王琳芳, 宋伟. 全转录组测序分析精子发生中RNA结合蛋白质的动态表达[J]. 基础医学与临床, 2021, 41(6): 825-830.

LI Kai, MANG Xin-yu, ZOU Ding-feng, LI Meng-zhen, MIAO Shi-ying, WANG Lin-fang, SONG Wei. Dynamic expression of RNA-binding proteins in spermatogenesis based on RNA-seq[J]. Basic & Clinical Medicine, 2021, 41(6): 825-830.

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