基础医学与临床 ›› 2021, Vol. 41 ›› Issue (3): 340-345.

• 研究论文 • 上一篇    下一篇

自身免疫性甲状腺炎小鼠的巨噬细胞极化失衡及代谢方式改变

王诗淇,赵蕾,史晓光   

  1. 中国医科大学附属第一医院
  • 收稿日期:2020-08-10 修回日期:2020-11-23 出版日期:2021-03-05 发布日期:2021-03-01
  • 通讯作者: 史晓光 E-mail:xiaoguangshi_cmu@163.com
  • 基金资助:
    自身免疫性甲状腺炎免疫代谢调节机制:糖酵解及其相关信号通路研究

Macrophage polarization imbalance and metabolic changes in autoimmune thyroiditis mice

  • Received:2020-08-10 Revised:2020-11-23 Online:2021-03-05 Published:2021-03-01
  • Supported by:
    Immuno-metabolic regulation in autoimmune thyroiditis: glycolysis and related signaling pathways

摘要: 目的 探讨巨噬细胞极化失衡及其代谢途径改变在自身免疫性甲状腺炎(AIT)发生发展中的意义。方法 将NOD.H-2h4雌鼠随机均分为对照组、AIT组(给予0.05%碘化钠水)。HE染色观察甲状腺组织淋巴细胞浸润情况并进行甲状腺炎性程度评分;酶联免疫吸附法(ELISA)测定血清甲状腺球蛋白抗体(TgAb);免疫荧光分析甲状腺组织中M1型和M2型巨噬细胞比例;Seahorse分析细胞能量代谢,测定细胞外酸化率(ECAR)、耗氧率(OCR)。结果 AIT组甲状腺组织有明显的淋巴细胞浸润,AIT的发病率明显高于对照组(P<0.05)。与对照组相比,AIT组小鼠甲状腺组织中M1型巨噬细胞占比增多,M2型占比减少(P<0.05);AIT组小鼠的最大ECAR和OCR值均明显高与对照组(P<0.05)。结论 巨噬细胞的极化失衡及其代谢改变对AIT的发生发展有着重要影响。

关键词: 关键词:自身免疫甲状腺炎, 免疫代谢, 巨噬细胞极化, 糖酵解, 代谢重编程

Abstract: Objective To investigate the significance of macrophage polarization imbalance and metabolic pathway changes in the occurrence and development of autoimmune thyroiditis (AIT).Methods 4 weeks old NOD.H-2h4 female rats were randomly divided into control group and AIT group(0.05% sodium iodide water). Hematoxylin-eosin staining was used to observe the lymphocytic infiltration of thyroid tissue. TgAb were measured by Enzyme Linked Immunosorbent Assay (ELISA). The ratio of M1 type and M2 type macrophages in thyroid tissue was analyzed by immunofluorescence. Seahorse was used to analyze the energy metabolism level of cells, and the extracellular acidification rate (ECAR) and O2 consumption rate (OCR) were measured. Results The thyroid tissue of mice in AIT group had obvious lymphocyte infiltration, and the incidence of AIT is significantly higher than the control group(P<0.05). Compared with the control group, the ratio of M1 and M2 macrophages in the thyroid tissue of mice in the AIT group increased, while the proportion of M2 type macrophages decreased (P<0.05); the ECAR and OCR values of mice in the high iodine drinking water group were significantly higher than those of the control group (P<0.05).Conclusion The change of macrophage metabolism and the polarization imbalance affected the occurrence and development of AIT.

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