苦参碱对人乳头状甲状腺癌细胞系TPC-1增殖和迁移的影响

基础医学与临床 ›› 2021, Vol. 41 ›› Issue (12): 1730-1735.

苦参碱对人乳头状甲状腺癌细胞系TPC-1增殖和迁移的影响

傅松波¹, 马承旭¹, 井高静¹, 赵楠¹, 汤旭磊^1*, 牟军勤², 常寅龙²

1.兰州大学第一医院内分泌科,甘肃兰州 730000;
2.兰州市城关区人民医院内分泌科,甘肃兰州 730030

收稿日期:2020-09-25 修回日期:2021-04-30 发布日期:2021-12-03
通讯作者: *xulei_tang@126.com
基金资助:
甘肃省省级科技计划项目(1004TCYA032);甘肃省兰州市城关区科技计划项目(2019SHFZ0038);甘肃省自然科学基金(20JR10RA681);甘肃省内分泌疾病临床医学研究中心建设方案项目(20JR10FA667)

Effect of matrine on proliferation and migration of human thyroid cancer cell line TPC-1

FU Song-bo¹, MA Cheng-xu¹, JING Gao-jing¹, ZHAO Nan¹, TANG Xu-lei^1*, MOU Jun-qin², CHANG Yin-long²

1. Department of Endocrinology, the First Hospital of Lanzhou University, Lanzhou 730000;
2. Department of Endocrinology,the People's Hospital of Chengguan District, Lanzhou 730030, China

Received:2020-09-25 Revised:2021-04-30 Published:2021-12-03
Contact: *xulei_tang@126.com

摘要/Abstract

摘要： 目的探讨苦参碱(matrine)下调miR-182-5p在人甲状腺乳头状癌细胞系(TPC-1)增殖和迁移中的作用。方法将实验分为:抑制剂对照组(inhibitor-NC)、miR-182-5p的拮抗剂组(inhibitor)、模拟物对照(mimic-NC)组、miR-182-5p 的模拟物组(miR-182-5p-mimics)、mimic-NC+苦参碱和miR-182-5p-mimics+苦参碱,TPC-1细胞系于转染24 h后分别加入或者不加入 0.5 mg/mL的苦参碱, PCR检测miR-182-5p、vinmentin、E-cadherin和N-cadherin基因表达,CCK-8法和Transwell小室法分别检测TPC-1细胞的增殖和迁移。Western blot检测vinmentin、E-cadherin和N-cadherin蛋白表达。结果在TPC-1细胞中,与Nthy-ori3-1(NT)相比, miR-182-5p的表达水平显著地增加(P＜0.01);抑制miR-182-5p的表达显著地抑制TPC-1细胞的增殖及迁移 (P＜0.01);与control组相比,剂量为0.5 mg/mL苦参碱能明显降低miR-182-5p的表达水平(P＜0.05)、降低增殖和迁移、下调N-cadherin及vinmentin表达水平,增加E-cadherin基因和蛋白的表达水平 (P＜0.01); 过表达miR-182-5p,减弱了苦参碱降低TPC-1细胞中vinmentin和N-cadherin的表达、增加E-cadherin的表达及抑制TPC-1细胞的增殖和迁移 (P＜0.01)。结论苦参碱可能是一种潜在的抗乳头状甲状腺癌(PTC)药物,miR-182可作为治疗PTC的有效靶标。

关键词: 苦参碱, miR-182-5p, 增殖, 迁移, 乳头状甲状腺癌

Abstract: Objective To investigate the mechanism of matrine inhibiting the proliferation and migration of thyroid cancer cell line TPC-1 through down-regulated miRNA-182-5p TPC-1. Methods TPC-1 cells were transfected after 24 hours with 0.5 mg/mL matrine or without 0.5 mg/mL matrine, the experiments were divided into: inhibitor-NC, inhibitor against miR-182-5p (inhibitor), mimic-NC, miRNA-182-5p-mimics, mimic-NC+matrine and miR-182-5p- mimics+matrine. miR-182-5p, E-cadherin, N-cadherin and vinmentin gene were detected by PCR. TPC-1 cells proliferation and migration were detected using CCK-8 and Transwell assay. The expression of E-cadherin、N-cadherin and vinmentin protein was determined by Western blot. Results Compared with Nthy-ori3-1 (NT), miR-182-5p was highly expressed in TPC-1 cells (P＜0.01); down-regulation to the expression of miRNA-182-5p inhibited the proliferation and migration of TPC-1 cells (P＜0.01); Compared to the 0 mg/mL matrine group, 0.5 mg/mL matrine significantly reduced the miR-182-5p level (P＜0.05) and inhibited proliferation, migration of TPC-1 cells and also reduced N-cadherin and vinmentin expression but increased E-cadherin expression in TPC-1 cells (P＜0.01); Over-expression of miR-182-5p partly abolished the matrine-inhibited proliferation and migration of TPC-1 cells and the increased E-cadherin expression, decreased miR-182-5p,N-cadherin and vinmentin expression (P＜0.01). Conclusions Matrine is a potential therapeutic drug for papillary thyroid carcinoma (PTC) and miR-182 may be an effective target for the treatment of PTC.

Key words: matrine, miR-182-5p, proliferation, migration, papillary thyroid cancer

中图分类号:

R736.1

傅松波, 马承旭, 井高静, 赵楠, 汤旭磊, 牟军勤, 常寅龙. 苦参碱对人乳头状甲状腺癌细胞系TPC-1增殖和迁移的影响[J]. 基础医学与临床, 2021, 41(12): 1730-1735.

FU Song-bo, MA Cheng-xu, JING Gao-jing, ZHAO Nan, TANG Xu-lei, MOU Jun-qin, CHANG Yin-long. Effect of matrine on proliferation and migration of human thyroid cancer cell line TPC-1[J]. Basic & Clinical Medicine, 2021, 41(12): 1730-1735.

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