炎性细胞因子和凋亡相关蛋白参与大鼠下肢深静脉血栓形成

基础医学与临床 ›› 2021, Vol. 41 ›› Issue (1): 50-54.

炎性细胞因子和凋亡相关蛋白参与大鼠下肢深静脉血栓形成

冯涛¹, 李晶², 潘金强¹, 郑殿宇¹, 陈辉¹, 杜曼·巴戈达提¹, 耿中利^1*

新疆医科大学附属中医医院 1.普外二科; 2.内分泌科,新疆乌鲁木齐 830000

收稿日期:2020-03-02 修回日期:2020-06-30 出版日期:2021-01-05 发布日期:2020-12-30
通讯作者: ^*46867442@qq.com
基金资助:
新疆维吾尔自治区自然科学基金(2019D01C167)

Inflammatory cytokines and apoptosis related proteins participate in the formation of deep vein thrombosis of lower extremity in rats

FENG Tao¹, LI Jing², PAN Jin-qiang¹, ZHENG Dian-yu¹, CHEN Hui¹, Duman BAGODAT¹, GENG Zhong-li^1*

1. Department of General Surgery; 2. Department of Endocrinology, the Affiliated Hospital of Traditional Chinese Medicine, Xinjiang Medical University, Urumqi 830000, China

Received:2020-03-02 Revised:2020-06-30 Online:2021-01-05 Published:2020-12-30
Contact: ^*46867442@qq.com

摘要/Abstract

摘要： 目的探讨IL-1β、TNF-α、Bcl-2和Bax在大鼠下腔深静脉血栓形成中的表达调控机制。方法将大鼠随机分为对照组(control)、下腔静脉血栓模型组(model)、miR-5189-3p抑制剂组(inhibitor)和miR-5189-3p抑制剂阴性对照(inhibitor NC)组,每组10只。24 h后,颈椎脱臼法处死,抽取血样和采集组织;用ELISA检测血样中IL-1β、TNF-α水平;病理学HE染色法观察下腔静脉血栓形态;Western blot检测下腔静脉组织Bcl-2,BAX蛋白的表达。结果模型组IL-1β和TNF-α含量较对照组显著升高(P＜0.05);miR-5189-3p抑制剂组较对照组显著升高(P＜0.05);miR-5189-3p抑制剂组较模型组显著升高(P＜0.05);miR-5189-3p抑制剂阴性对照较模型组显著降低(P＜0.05)。模型组和miR-5189-3p抑制剂组可见红色血栓和混合血栓,血管壁伴炎性细胞浸润,miR-5189-3p抑制剂阴性对照血栓明显机化,有血流再通的征象。模型组Bcl-2/ BAX蛋白表达较对照组显著降低(P＜0.05);miR-5189-3p抑制剂组较模型组显著降低(P＜0.05);miR-5189-3p抑制剂阴性对照较模型组显著升高(P＜0.05)。结论 miR-5189-3p可以通过调节炎性细胞因子和凋亡相关蛋白表达,从而影响下肢深静脉血栓的形成,为后期研究提供了新的思路与基础。

关键词: 深静脉血栓, 静脉内皮细胞, Bcl-2, BAX

Abstract: Objective To investigate the expression and regulation mechanism of IL-1β, TNF-α, Bcl-2 and BAX in rats with inferior vena cava thrombosis. Methods The rats were randomly divided into control group, inferior vena cava thrombus model group, mir-5189-3p inhibitor group and miR-5189-3p inhibitor NC group. Twenty-four hours later, the animals were killed by cervical vertebrae dislocation, blood samples were taken and tissues were collected; the level of IL-1β and TNF-α in blood samples were detected by ELISA; The morphology of inferior vena cava thrombus was microscopied with HE staining; The expression of Bcl-2 and BAX protein in inferior vena cava was detected by Western blot. Results The level of IL-1β and TNF-α in the model group was significantly higher than those in the control group (P＜0.05); the level of miR-5189-3p inhibitor group was significantly higher than that in the control group (P＜0.05); the level of miR-5189-3p inhibitor group was significantly higher than that in the model group (P＜0.05); the level of miR-5189-3p inhibitor negative control group was significantly lower than that in the model group (P＜0.05). In the model group and miR-5189-3p inhibitor group, red thrombus and mixed thrombus were found; the vessel showed inflammatory cell infiltration, and thrombus in the negative control group of miR-5189-3p inhibitor was obviously organized, with signs of blood flow recanalization. Bcl-2/BAX protein level in the model group was significantly lower than that in the control group (P＜0.05); miR-5189-3p inhibitor group was significantly lower than that in the model group (P＜0.05); while miR-5189-3p inhibitor in negative control group was significantly higher than that in the model group (P＜0.05). Conclusions miR-5189-3p can affect the formation of DVT by regulating the expression of inflammatory cytokines and apoptosis related proteins, which provides a new idea and basis for later research.

Key words: deep vein thrombosis, vein endothelial cells, Bcl-2, BAX

中图分类号:

R364.1+5

冯涛, 李晶, 潘金强, 郑殿宇, 陈辉, 杜曼·巴戈达提, 耿中利. 炎性细胞因子和凋亡相关蛋白参与大鼠下肢深静脉血栓形成[J]. 基础医学与临床, 2021, 41(1): 50-54.

FENG Tao, LI Jing, PAN Jin-qiang, ZHENG Dian-yu, CHEN Hui, Duman BAGODAT, GENG Zhong-li. Inflammatory cytokines and apoptosis related proteins participate in the formation of deep vein thrombosis of lower extremity in rats[J]. Basic & Clinical Medicine, 2021, 41(1): 50-54.

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