关键词: PTEN沉默, 氧化应激, 心机细胞损伤

Abstract: Objective To investigate the effect and mechanism of phosphatase and tensin homolog deleted on chromosome ten (PTEN) silencing on cardiomyocyte induced by H2O2 injury in rats. Methods The isolated rat cardiomyocytes were divided into blank control group and H2O2 treated group. (50, 100 and 200 μmol/L), and the LDH release of cardiomyocytes was detected by kit, apoptosis of cardiomyocytes was detected by flow cytometry, and the expression of PTEN was detected by RT-qPCR and Western blot. The expression of PTEN were detected by RT-qPCR and Western blot after transfection of siR-PTEN. The effect of siR-PTEN on LDH release of cardiomyocytes induced by oxidative stress was detected by kit, the effect of siR-PTEN on cardiomyocyte apoptosis induced by oxidative stress was detected by flow cytometry. Results Compared with the control group, the LDH release and apoptosis rate of myocardial cells increased significantly (P < 0.05) and the expression of PTEN mRNA and protein increased significantly (P < 0.05) after H2O2 treatment. After transfection of siR-PTEN, the LDH release rate and apoptosis rate of cardiomyocytes were significantly decreased (P<0.05). Conclusion PTEN silencing could inhibit the injury of cardiomyocytes induced by oxidative stress.

Key words: PTEN silencing, oxidative stress, cardiac cell damage