沉默PTEN基因抑制H2O2诱导的大鼠心肌细胞损伤

基础医学与临床 ›› 2019, Vol. 39 ›› Issue (5): 705-709.

沉默PTEN基因抑制H2O2诱导的大鼠心肌细胞损伤

王晓景¹,张明星²,陈小亮³

1. 武汉市普仁医院
2. 湖北省武警总队医院
3. 湖南省郴州市第一人民医院

收稿日期:2018-08-28 修回日期:2019-01-24 出版日期:2019-05-05 发布日期:2019-04-24
通讯作者: 陈小亮 E-mail:2290304514@qq.com
基金资助:
郴州市科技局项目

Silencing PTEN gene inhibits cardiomyocyte injury induced by H2O2 in rats

Received:2018-08-28 Revised:2019-01-24 Online:2019-05-05 Published:2019-04-24

摘要/Abstract

摘要： 目的探究肿瘤抑制因子沉默PTEN后对H2O2诱导的大鼠心肌细胞损伤的作用及其机制。方法将培养的大鼠心肌细胞分为对照组和H2O2处理组（50、100和200 μmol/L）后，试剂盒检测心肌细胞乳酸脱氢酶（LDH）释放及氧化应激诱导后心肌细胞LDH释放量；流式细胞检测术检测心机细胞凋亡；RT-qPCR和Western blot检测心肌细胞PTEN表达水平及转染siR-PTEN后，RT-qPCR和Western blot检测转染效率；流式细胞检测术检测siR-PTEN在氧化应激诱导后心肌细胞凋亡。结果与对照组相比，H2O2显著增加心肌细胞LDH释放（P<0.05），细胞凋亡率显著上升（P<0.05）、PTEN mRNA和蛋白表达显著上调（P<0.05），且呈浓度依赖性；siR-PTEN促进心肌细胞LDH释放和降低细胞凋亡率（P<0.05）。结论 PTEN沉默表达可抑制氧化应激反应导致的大鼠心肌细胞损伤。

关键词: PTEN沉默, 氧化应激, 心机细胞损伤

Abstract: Objective To investigate the effect and mechanism of phosphatase and tensin homolog deleted on chromosome ten (PTEN) silencing on cardiomyocyte induced by H2O2 injury in rats. Methods The isolated rat cardiomyocytes were divided into blank control group and H2O2 treated group. (50, 100 and 200 μmol/L), and the LDH release of cardiomyocytes was detected by kit, apoptosis of cardiomyocytes was detected by flow cytometry, and the expression of PTEN was detected by RT-qPCR and Western blot. The expression of PTEN were detected by RT-qPCR and Western blot after transfection of siR-PTEN. The effect of siR-PTEN on LDH release of cardiomyocytes induced by oxidative stress was detected by kit, the effect of siR-PTEN on cardiomyocyte apoptosis induced by oxidative stress was detected by flow cytometry. Results Compared with the control group, the LDH release and apoptosis rate of myocardial cells increased significantly (P < 0.05) and the expression of PTEN mRNA and protein increased significantly (P < 0.05) after H2O2 treatment. After transfection of siR-PTEN, the LDH release rate and apoptosis rate of cardiomyocytes were significantly decreased (P<0.05). Conclusion PTEN silencing could inhibit the injury of cardiomyocytes induced by oxidative stress.

Key words: PTEN silencing, oxidative stress, cardiac cell damage

王晓景张明星陈小亮. 沉默PTEN基因抑制H2O2诱导的大鼠心肌细胞损伤[J]. 基础医学与临床, 2019, 39(5): 705-709.

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