关键词: VCAM-1, ox-LDL, HUVECs, 氧化应激

Abstract: Objective To investigate the effect of vascular cell adhesion molecule-1 (VCAM-1) on oxidative low density lipoprotein (ox-LDL)-induced oxidative damage and apoptosis of human venous endothelial cells (HUVECs). Methods HUVECs cultured in vitro were randomly divided into control group, ox-LDL group, siRNA-NC group, and siRNA-VCAM-1 group. After HUVECs were transfected with siRNA-NC or siRNA-VCAM-1 by Lipofectamine 2000, 100μg/mL ox-LDL was added for 24h. Western Blot was used to detect the level of VCAM-1 protein; The viability of lung cancer cells was measured by thiazolium (MTT) assay; The cell apoptosis rate and reactive oxygen species (ROS) level were analyzed by flow cytometry, and the kit was used to detect malondialdehyde (MDA) and superoxide dismutase (SOD) levels. Results The results showed that VCAM-1 expression was up-regulated in ox-LDL-induced HUVECs（P<0.05）; siRNA-VCAM-1 significantly decreased VCAM-1 protein levels in ox-LDL-induced HUVECs（P<0.05）; Down-regulation of VCAM-1 could increase cell viability（P<0.05）, decreased apoptotic rate, ROS and MDA levels（P<0.05）, increased SOD levels（P<0.05）. Conclusions Down-regulation of VCAM-1 can increase the activity of HUVECs, reduce apoptosis, and inhibit oxidative stress.

Key words: VCAM-1, ox-LDL, HUVECs, Oxidative stress