基础医学与临床 ›› 2018, Vol. 38 ›› Issue (2): 185-188.
• 研究论文 • 上一篇 下一篇
吕文秀1,吴文韬2,郑士梅1,谢林虎3,丁伯平3
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摘要: 目的 探究姜黄素对顺铂诱导凋亡的人肾小管上皮细胞(HK-2)的影响。方法 用MTT法观察HK-2增殖;用Western blot检测HK-2的凋亡蛋白。结果 1)顺铂呈剂量依赖性诱导HK-2凋亡(P<0.05)。2)在姜黄素和顺铂联合作用下,凋亡的HK-2明显减少,细胞存活率提高(P<0.05);HK-2中Bax蛋白表达降低(P<0.05);Bcl-2蛋白表达增加 (P<0.05)。结论 姜黄素可以通过调控Bax和Bcl-2蛋白的表达抑制顺铂引起的细胞凋亡。
关键词: 姜黄素, 顺铂, 人肾上皮细胞, 细胞凋亡
Abstract: Objective To investigate whether curcumin could inhibit cisplatin-induced apoptosis of HK-2 cells. Methods Using MTT methods to observe inhibitory effects of curcumin and cisplatin on the proliferation of HK-2 cell. Detecting caspases expression after curcumin and cisplatin treatment by Western blot. Results To some extent, cisplatin can induce cell apoptosis, and its effect is dose-dependent(P<0.05).Under the joint effect of curcumin and cisplatin on HK-2 cell,the number of apoptosis decreased(P<0.05), yet in the same time the expressions of Bcl-2 protein were increased(P<0.05) and the expressions of Bcl-2 protein were decreased(P<0.05). Conclusion Cisplatin enhances the apoptosis of HK-2 cell in a dose-dependent relationship within limits, and possess the antagonistic effect when cisplatin and curcumin are conbined. Curcumin can antagonize cisplatin-induced apoptosis in HK-2 by regulating the expressions of Bax and Bcl-2 protein.
Key words: curcumin , cisplatin, HK-2 cell, apoptosis
吕文秀 吴文韬 郑士梅 谢林虎 丁伯平. 姜黄素抑制顺铂诱导人肾小管上皮细胞系HK-2的凋亡[J]. 基础医学与临床, 2018, 38(2): 185-188.
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http://journal11.magtechjournal.com/Jwk_jcyxylc/CN/Y2018/V38/I2/185