长链非编码 RNA EVADR 促进人结直肠癌细胞系增殖及迁移

基础医学与临床 ›› 2018, Vol. 38 ›› Issue (2): 174-179.

长链非编码 RNA EVADR 促进人结直肠癌细胞系增殖及迁移

韩丹¹,童亚楠²,贾银平²,唐梦灵²,曹刘素²,张竹君³,潘渠¹

1. 成都医学院
2. 陆军军医大学
3. 第三军医大学第一附属医院

收稿日期:2017-08-31 修回日期:2017-12-05 出版日期:2018-02-05 发布日期:2018-01-24
通讯作者: 潘渠 E-mail:ppqlove@126.com
基金资助:
国家自然科学基金

lncRNA EVADR promotes proliferation and migration of human colorectal cancer cell lines

Received:2017-08-31 Revised:2017-12-05 Online:2018-02-05 Published:2018-01-24

摘要/Abstract

摘要： 目的探讨 LncRNA EVADR 对人结直肠癌 HCT116和LOVO 细胞系的增殖和迁移的影响以及其作用机制。方法利用慢病毒感染方式分别构建稳定过表达 LncRNA EVADR 的 HCT116和LOVO 细胞株；用 CCK-8 检测细胞的增殖；Transwell 小室法检测细胞迁移能力，Western blot 检测 E-cadherin 的表达，实时荧光定量 PCR 检测 Snail、Slug、ZEB1和ZEB2 mRNA的表达。结果成功构建了稳定过表达LncRNA EVADR的结直肠癌 HCT116和LOVO 细胞株。与 HCT116-NC和LOVO-NC 细胞组对比，HCT116-EVADR与LOVO-EVADR 细胞组的增殖和迁移能力明显提高（P<0.05）。同时，上皮标志物 E-cadherin 表达明显降低，而 Snail、Slug、ZEB1和ZEB2 间质化标志物的表达升高。结论过表达 LncRNA EVADR 具有促进结直肠癌 HCT116和LOVO 细胞的增殖与迁移能力，可能通过调节结直肠癌细胞上皮间质转化（EMT）发挥作用。

关键词: 关键词：结直肠癌, LncRNA EVADR, 增殖, 迁移

Abstract: Objective To explore the effect and mechanism of lncRNA EVADR on the proliferation and migration in human colorectal cancer cell lines HCT116 and LOVO. Methods HCT116 and LOVO cell lines were transfected with LncRNA EVADR by overexpressing lentivirus system. CCK8 assay was performed to measure the growth of HCT116 and LOVO cells after overexpression of EVADR. Transwell migration was performed to determine if EVADR could promote HCT116 and LOVO cells migration. Finally, the expressions of E-cadherin and transcription factor Snail, Slug, ZEB1 and ZEB2 were detected by Western blot and real - time quantitative PCR respectively. Results We successfully established colorectal cancer cells strains HCT116, LOVO which can stably overexpress LncRNA EVADR and the capacity of proliferation and migration in overexpression group were significantly improved (P <0.05). The expression of E-cadherin was decreased while mesenchymal markers Snail, Slug, ZEB1 and ZEB2 were increased in EVADR overexpression HCT116 and LOVO cells. Conclusion Overexpression of LncRNA EVADR in HCT116 and LOVO cells can significantly promote the proliferation and migration of HCT116 and LOVO cells, which may play a important role in regulating epithelial-mesenchymal transition in colorectal cancer cells.

Key words: Key words: Colorectal cancer, LncRNA EVADR, Proliferation, migration

中图分类号:

R735

韩丹童亚楠贾银平唐梦灵曹刘素张竹君潘渠. 长链非编码 RNA EVADR 促进人结直肠癌细胞系增殖及迁移[J]. 基础医学与临床, 2018, 38(2): 174-179.

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