基础医学与临床 ›› 2017, Vol. 37 ›› Issue (9): 1303-1307.

• 研究论文 • 上一篇    下一篇

紫杉醇联合奥沙利铂对非小细胞肺癌模型小鼠的治疗作用

毛宁,熊弢   

  1. 重庆医科大学附属永川医院
  • 收稿日期:2016-08-02 修回日期:2016-11-23 出版日期:2017-09-05 发布日期:2017-08-28
  • 通讯作者: 熊弢 E-mail:kw2111@163.om

Treatment of paclitaxel plus oxaliplatin chemotherapy to the non-small cell lung cancer mouse model

  • Received:2016-08-02 Revised:2016-11-23 Online:2017-09-05 Published:2017-08-28

摘要: 目的 观察紫杉醇联合奥沙利铂对荷瘤裸鼠非小细胞肺癌治疗效果以及对PDCD5和XIAP表达的影响。方法 制备裸鼠肺癌荷瘤模型,将荷瘤小鼠随机分为空白组、0.9% NaCL溶液组、奥沙利铂组、紫杉醇组和紫杉醇联合奥沙利铂组,q-PCR检测各组PDCD5和XIAP基因表达水平,Western bolt分析各组PDCD5和XIAP蛋白表达情况,对比分析各组肿瘤组织重量。 结果 紫杉醇联合奥沙利铂组PDCD5 mRNA表达水平最高(P<0.01),XIAP mRNA表达水平最低(P<0.01);紫杉醇联合奥沙利铂组PDCD5蛋白表达最高(P<0.01),XIAP蛋白表达最低(P<0.01)。对比各分组肿瘤组织重量,紫杉醇联合奥沙利铂组肿瘤质量最小(P<0.01)。 结论 紫杉醇联合奥沙利铂化疗能显著增加PDCD5表达和降低 XIAP表达,能使已发生的非小细胞肺癌组织质量显著减少。

关键词: 紫杉醇, 奥沙利铂, 非小细胞肺癌, 细胞凋亡, 程序性细胞死亡蛋白-5(PDCD5), X-连锁凋亡抑制蛋白(XIAP)

Abstract: Objective To investigate the therapeutic effect of paclitaxel plus oxaliplatin chemotherapy to the transplanted non-small cell lung cancer of nude mice and the effect to the apoptosis protein expression of PDCD5 and XIAP. Methods Building the nude mice model of transplanted lung cancer and all tumor-bearing mice were randomly divided into blank group, normal saline group, oxaliplatin group, paclitaxel group, paclitaxel plus oxaliplatin group. The gene expression of PDCD5 and XIAP were assayed by real-time quantitative PCR (q-PCR). The apoptosis related PDCD5 and XIAP protein expression was detected by Western blot. Finally, the tumor weight of each group was measured for statistical analysis. Results The mRNA expression of PDCD5 was highest and the gene expression of XIAP was lowest in paclitaxel plus oxaliplatin group (P < 0.01). The expression of PDCD5 protein was highest and the expression of XIAP protein was lowest in paclitaxel plus oxaliplatin group (P < 0.01). Finally, compare the tumor weight of each group, paclitaxel plus oxaliplatin group has the least mass (P < 0.01). Conclusion: Paclitaxel plus oxaliplatin group chemotherapy can significantly increase PDCD5 expression and reduce XIAP expression. Meanwhile, paclitaxel plus oxaliplatin chemotherapy can significantly reduce the tumor weight of happened non-small cell lung cancer.

Key words: Paclitaxel, Oxaliplatin, Non-small cell lung cancer, Cell apoptosis, Programmed Cell Death 5(PDCD5), X-linked inhibitor of apoptosis protein(XIAP)