mTOR负调控miR-27a并通过靶向降低GP73抑制人肝癌细胞侵袭

基础医学与临床 ›› 2017, Vol. 37 ›› Issue (7): 1015-1020.

mTOR负调控miR-27a并通过靶向降低GP73抑制人肝癌细胞侵袭

盖晓晨¹,汤步富²,刘芳铭¹,吴玉婷¹,王芳³,张宏冰⁴

1. 中国医学科学院基础医学研究所
2. 大连医科大学附属第一医院
3. 中国医学科学院基础医学研究所北京协和医学院基础学院
4. 中国医学科学院基础所生理和病理生理系

收稿日期:2017-04-19 修回日期:2017-05-16 出版日期:2017-07-05 发布日期:2017-06-23
通讯作者: 刘芳铭 E-mail:jjlfmzhy@sina.com
基金资助:
中国医学科学院医学与健康科技创新工程

miR-27a is negatively regulated by mTOR and inhibits liver cancer cell invasion via targeting GP73

Received:2017-04-19 Revised:2017-05-16 Online:2017-07-05 Published:2017-06-23

摘要/Abstract

摘要： 目的探索mTOR促进肝癌细胞侵袭能力机理。方法使用q-PCR方法检测miR-27a和GP73表达；将miR-27a的mimic转染GP73高表达的M97H细胞中，并将miR-27a inhibitor转入GP73低表达HepG2细胞中，q-PCR和Western blot观察GP73的表达；使用荧光素酶报告基因系统验证miR-27a在GP73的3UTR区的结合位点；将miR-27a mimic转染GP73高表达的M97H细胞和GP73低表达的HepG2细胞，Transwell实验观察细胞的侵袭。结果 mTOR下调miR-27a，并上调GP73的表达；miR-27a下调GP73的表达，抑制miR-27a则上调GP73；GP73是miR-27a的靶基因；miR-27a显著抑制M97H细胞的侵袭，但对HepG2细胞无抑制效果。结论：mTOR负调控的miR-27a靶向GP73抑制肝癌细胞的侵袭。

关键词: 关键词：肝癌, mTOR, GP73, 侵袭

Abstract: Objective This study aims to explore the mechanism of mTOR-mediated liver cancer cell invasion. Methods q-PCR was used to check the expression of miR-27a and GP73; miR-27a mimics were transfected into GP73-high expressing M97H cells and miR-27a inhibitors were transfected into GP73-low expressing HepG2 cells, q-PCR and Western blot were performed to observe the expression of GP73; Dual-luciferase assay was also performed to verify the binding sites of miR-27a in GP73 3’UTR; miR-27a mimics were transfected into M97H cells and miR-27a inhibitors were transfected into HepG2 cells, Transwell assay was used to measure cell invasion. Results mTOR downregulated miR-27a and upregulated GP73; GP73 was downregulated by miR-27a and upregulated by miR-27a suppression; GP73 was a target gene of miR-27a; miR-27a inhibited the invasion of M97H cells rather than HepG2 cells. Conclusions miR-27a is negatively regulated by mTOR and inhibits liver cancer cell invasion via targeting GP73.

Key words: Key words: Liver Cancer, mTOR, GP73, invasion

中图分类号:

盖晓晨汤步富刘芳铭吴玉婷王芳张宏冰. mTOR负调控miR-27a并通过靶向降低GP73抑制人肝癌细胞侵袭[J]. 基础医学与临床, 2017, 37(7): 1015-1020.

[1]	陈民佳杜娟袁丹凤张蜀黄宏朱方强. 姜黄素抑制TGF-β1诱导的人肾小管上皮细胞系表型转化[J]. , 2020, 40(1): 48-53.
[2]	孙光炎丁明炎姚和迁曾凡杨. HMG-CoA2裂解酶在鼻咽癌组织中表达及其对鼻咽癌细胞增殖、侵袭能力的影响[J]. 基础医学与临床, 2019, 39(9): 1294-1299.
[3]	武永强杨帆. 干扰Arf6抑制人前列腺癌细胞系DU145增殖、迁移及侵袭[J]. 基础医学与临床, 2019, 39(9): 1310-1315.
[4]	张杨杜雯雯刘婷曾园园黄建安. miR-195-5p表达上调抑制肺癌细胞系A549的迁移和侵袭[J]. , 2019, 39(12): 1694-1698.
[5]	刘雅林谢兰兰王丽云赵瑞杰许峰王文胜. 丁苯酞减轻阿尔兹海默病大鼠海马神经元凋亡[J]. , 2019, 39(12): 1746-1751.
[6]	施能杨欣谕. miR-195抑制舌鳞癌细胞系CAL27增殖、迁移和侵袭[J]. , 2019, 39(12): 1689-1693.
[7]	陈素华马天江张智慧张磊史磊. 淫羊藿苷通过下调转移相关蛋白2抑制MGC803细胞的增殖、迁移和侵袭[J]. , 2019, 39(11): 1587-1591.
[8]	王盛华付民赵高峰. 七氟烷抑制人乳腺癌细胞系MCF-7增殖、迁移和侵袭[J]. , 2019, 39(11): 1597-1602.
[9]	韩俊岭陈昆郭亮马曜辉韩前河单中杰宋东奎. 下调uPAR表达抑制高侵袭性人膀胱癌细胞系T24的迁移与侵袭?[J]. , 2019, 39(11): 1568-1573.
[10]	方复毛华辉卢国春郭渊童向美. IL-8在胃癌组织上皮间质转化过程中表达增强[J]. 基础医学与临床, 2019, 39(10): 1444-1448.
[11]	刘桂元张永慧庞毅. WNT信号抑制因子DKK3基因对结直肠癌细胞系HCT116迁移与侵袭的影响[J]. 基础医学与临床, 2019, 39(10): 1437-1443.
[12]	涂其武沈晓黎. miR-34a通过MMP2抑制脑胶质瘤细胞侵袭和转移[J]. 基础医学与临床, 2018, 38(8): 1115-1120.
[13]	荆小马王全全郝延磊. 肌肉衰减综合征小鼠腓肠肌Sirt1和mTOR活性的变化[J]. 基础医学与临床, 2018, 38(7): 922-927.
[14]	张达容王维莲吴立翔柳满然. Twist促进人三阴性乳腺癌细胞迁移和侵袭[J]. 基础医学与临床, 2018, 38(6): 771-775.
[15]	张洋张静季琳李文媛王莹孙平于伟光. TRIM11靶向调控miR-24-3p促进乳腺癌MCF7细胞系增殖和侵袭[J]. 基础医学与临床, 2018, 38(4): 451-457.