关键词: MiRNA-144, 卵巢癌, Beclin-1, 细胞自噬

Abstract: Objective To detect the influence of rapamycin on the expression of 4 kinds of miRNAs about cell autophagy.To study the the relationship of miR-144 and Beclin-1 gene. Methods SKOV-3 cells were treated 50ng/mL rapamycin 2 hours and 10nmol/L 3-methyl adenine 12 hours,the expression of miR-17,miR-20a,miR-144 and miR-155 was detected by RT-qPCR in SKOV-3 cell of different groups，the protein expression of Beclin-1 was detected by Western blot.The targeting effect of miR-144 on Beclin-1 gene was verified by the dual-luciferase reporter assay,Western blot and RT-qPCR.Results The expression of miR-17,miR-144 and miR-155 were increased compared with NC groups in rapamycin group (P<0.05); miR-17,miR-20a and miR-144 were down regulated compared with NC group in 3-MA group(P<0.05)；the protein of Beclin-1 was down expression compared with NC group in rapamycin group.miR-144 could suppress Beclin-1 expression by targeting the specific 3’ untranslated region sequence of Beclin-1 gene. Conclusions miR-144 can directly inhibit the autophagy-related gene Beclin-1 expression and participate in the regulation of autophagy process in SKOV-3 cells.

Key words: MiRNA-144, Ovarian cancer, Beclin-1, Cell autophagy