ATAD2对人胶质瘤细胞系转移及上皮间充质转换的影响

基础医学与临床 ›› 2017, Vol. 37 ›› Issue (4): 512-517.

ATAD2对人胶质瘤细胞系转移及上皮间充质转换的影响

秦彦昌¹,贾栋²,于庆伟¹

1. 第四军医大学唐都医院
2. 第四军医大学唐都医院神经外科

收稿日期:2016-05-23 修回日期:2016-07-10 出版日期:2017-04-05 发布日期:2017-03-24
通讯作者: 秦彦昌 E-mail:wenyinxian029@163.com

Effect of ATAD2 on metastasis and epithelial-mesenchymal transition of human glioblastoma cell lines

Received:2016-05-23 Revised:2016-07-10 Online:2017-04-05 Published:2017-03-24

摘要/Abstract

摘要： 目的探讨三磷酸腺苷酶家族蛋白2（ATAD2）对人胶质瘤细胞的影响。方法用real-time PCR及Western blot检测ATAD2在人胶质瘤细胞系U87MG、U251和正常人星形胶质细胞的表达。U87MG和U251分为对照组（control）、脂质体对照组（mock）、ATAD2 siRNA（转染特异性siRNA）和ATAD2 overexpression（过表达ATAD2）4组，MTT法、Transwell实验观察细胞增殖和侵袭迁移。Western blot检测上皮间质转换标志蛋白上皮钙黏素、神经钙黏素和波形蛋白的表达。结果 U87MG和U251细胞ATAD2高表达。与对照相比，ATAD2 siRNA抑制U87MG和U251细胞增殖、侵袭、迁移、神经钙黏素及波形蛋白表达，促进上皮钙黏素表达（P<0.05）；ATAD2 overexpression促进细胞增殖、侵袭、迁移、神经钙黏素和波形蛋白表达，降低上皮钙黏素表达（P<0.05）。结论 ATAD2可能通过调节上皮间充质转换进程参与人胶质瘤细胞转移。

关键词: 三磷酸腺苷酶家族蛋白2, 胶质瘤 , 侵袭, 迁移, 上皮间充质转换

Abstract: Objective To analyze the effect of ATAD2 on glioma cell lines. Methods ATAD2 levels in U87MG, U251 and normal human astrocytes were detected by real-time PCR and Western blot. U87MG and U251 cells were divided into four groups: control, mock (lipofection control), ATAD2 siRNA (transfected with ATAD2 siRNA) and ATAD2 overexpression (transfected with ATAD2). Cell proliferation, migration and invasion were respectively measured by MTT and Transwell assay. The levels of E-cadherin, N-cadherin and Vimentin were measured by Western blot. Results ATAD2 was highly expressed in U87MG and U251 cells. Compared with control, cell proliferation, invasion, migration, N-cadherin and Vimentin expression were decreased by ATAD2 siRNA, while they were promoted by ATAD2 overexpression (P<0.05). E-cadherin expression was upregulated by ATAD2 siRNA and it was inhibited by ATAD2 overexpression (P<0.05). Conclusion ATAD2 participants in human glioma cells metastasis via epithelial-mesenchymal transition (EMT).

Key words: invasive power, neuron

秦彦昌贾栋于庆伟. ATAD2对人胶质瘤细胞系转移及上皮间充质转换的影响[J]. 基础医学与临床, 2017, 37(4): 512-517.

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