关键词: KIAA1456, 基因芯片, 慢病毒载体, RNA干扰, lncRNAs

Abstract: Objective Exploring the potential lncRNAs of KIAA1456 inhibiting ovarian cancer cells . Methods Immunofluorescence was used to detect the expression of KIAA1456 in HO8910PM ovarian cancer cell. Microarray was performed to identify differently expressed lncRNAs in HO8910PM ovarian cancer cells overexpressed KIAA1456 compared with the normal control group． The interesting candidate lncRNAs were verified by real-time quantitative PCR．Constructing RNAi lentiviral vector targeting KIAA1456 gene in HO8910PM ovarian cancer cells, RT-qPCR and Western blot was performed to measure the interference efficiency. Differentially expressed lncRNAs were validated by RT－qPCR after KIAA1456 knock-down. Results KIAA1456 mainly expressed in nucleus of HO8910PM cell. A total of 654 differently expressed lncRNAs were identified in the KIAA1456-overexpressed group compared with the control group with a set filter fold-change ≥1.2, the most aberrantly expressed lncRNAs including down-regulated gene SSX8 and up-regulated genes SNORD14D,SNORA26，lncRNA-ENST00000384488. The results of the RT-qPCR were consistent with the data from the microarray．The shRNA interference vectors targeting the KIAA1456 gene were successfully constructed, SSX8 was up-regulated, and SNORD14D,SNORA26，lncRNA-ENST00000384488 were down-regulated after knockdown the expression of KIAA1456 in the HO8910/PM ovarian cancer cells. Conclusions KIAA1456 could inhibit the proliferation of ovarian cancer by regulating correlative lncRNAs, such as SSX8,SNORD14D,SNORA26，lncRNA-ENST00000384488, which could provide a new target for the diagnosis and treatment of ovarian cancer.

Key words: KIAA1456, Microarray, Lentiviral vector, RNA interference, lncRNAs