PTPN13在人胃癌组织及胃癌SGC-7901细胞系中的表达及其对细胞增殖和侵袭的影响

基础医学与临床 ›› 2017, Vol. 37 ›› Issue (3): 376-381.

PTPN13在人胃癌组织及胃癌SGC-7901细胞系中的表达及其对细胞增殖和侵袭的影响

张静¹,冯颖¹,王芳²

1. 兰州大学第一医院
2. 兰州大学第一医院病理科

收稿日期:2016-03-09 修回日期:2016-07-05 出版日期:2017-03-05 发布日期:2017-02-23
通讯作者: 张静 E-mail:zhangjlzu@yeah.net

Expression of PTPN13 in human gastric cancer and gastric cancer SGC-7901 cell line and its association with proliferation and invasion

Received:2016-03-09 Revised:2016-07-05 Online:2017-03-05 Published:2017-02-23

摘要/Abstract

摘要： 目的分析PTPN13在人胃癌组织中及胃癌SGC-7901细胞系中的表达及其对细胞增殖、侵袭的影响。方法收集106例胃癌患者的胃癌组织及癌周正常组织标本。常规培养胃癌SGC-7901细胞并分为pcDNA3.1-PTPN13质粒转染组和未转染组。免疫组织化学法检测组织中的PTPN13表达。分析PTPN13表达与患者性别、年龄、肿瘤部位、肿瘤大小、浸润深度及转移的关系。Kaplan-Meier曲线分析不同PTPN13表达患者生存率的差异。CCK-8法检测细胞的增殖能力，Trans-well试验分析侵袭能力，Western blot检测E-cadherin、Snail及MMP9的表达。结果胃癌组织中的PTPN13阳性率低于癌周正常胃组织（31% vs.83%，P<0.05）。PTPN13的表达与肿瘤直径、浸润深度、淋巴结和远隔器官转移情况有关（P均＜0.05 ）。PTPN13阴性的胃癌患者2生存率较低。PTPN13过表达可以降低SGC-7901细胞的增殖率（P<0.05），同时降低其侵袭能力（P<0.05）。上调PTPN13后SGC-7901细胞上皮化标志物E-cadherin表达增加，而间质化标志物Snail和MMP9表达减少。结论 PTPN13在胃癌组织和胃癌细胞中具有肿瘤抑制作用，较低的PTPN13表达提示患者预后不良。PTPN13具有成为胃癌的治疗诊断或治疗靶点的潜力。

关键词: 胃癌, 非受体蛋白酪氨酸磷酸酶-13, 增殖, 侵袭

Abstract: Objective: to investigate the expression of PTPN13 in human gastric cancer and gastric cancer SGC-7901 cell line and its association with proliferation and invasion. Methods: 106 cases gastric cancer tissue samples and matched normal peritumorial tissues were collected. SGC-7901 cells were cultured and divided into two groups including pcDNA3.1-PTPN13 transfection group and without transfection group. Immunohistochemical technique were used to detected protein expression of PTPN13. The association of PTPN13 expression with tumor location, tumor size, depth of invasion and tumor metastasis was analyzed. The survival rate of patients with different PTPN13 expression was calculated by Kaplan-Meier curves. CCK-8 assay was used to estimate the proliferation changes. Using Trans-well assay analyzed the invasion of SGC-7901 cells. Moreover, Western blot was performed to detect the markers of EMT including E-cadherin, Snail and MMP9.Results: Immunohistochemistry showed the expression rate of PTPN13 in gastric cancer tissues was lower than normal tissue (31 % vs.83%, P<0.05). The expression of PTPN13 in patients was related to with different tumor size, depth of invasion and tumor metastasis (P<0.05). The 2-years survival rate of patients with negative PTPN13 expression were lower. Overexpression reduced both proliferation and invasion in SGC-7901 cells. Western blot showed that up-regulated PTPN13 could increase E-cadherin level but decreased the level of Snail and MMP9.Conclusions: PTPN13 play a role in gastric cancer tissue and cells as a tumor suppressor. Lower PTPN13 may indicate a poor prognosis. PTPN13 could be used as therapy target in gastric cancer.

Key words: Gastric Cancer, Protein Tyrosine Phosphatases Non-Receptor Type 13, Proliferation, Invasion

张静冯颖王芳. PTPN13在人胃癌组织及胃癌SGC-7901细胞系中的表达及其对细胞增殖和侵袭的影响[J]. 基础医学与临床, 2017, 37(3): 376-381.

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