基础医学与临床 ›› 2016, Vol. 36 ›› Issue (12): 1630-1635.

• 研究论文 • 上一篇    下一篇

亚硒酸钠诱导结直肠癌SW480细胞系凋亡

唐振东1,慈雅丽2,杨洋3,许彩民1   

  1. 1. 北京协和医学院基础学院
    2. 中国医学科学院 北京协和医学院
    3. 中国医学科学院基础医学研究所
  • 收稿日期:2016-09-18 修回日期:2016-10-22 出版日期:2016-12-05 发布日期:2016-11-29
  • 通讯作者: 许彩民 E-mail:cmxu@ibms.pumc.edu.cn
  • 基金资助:
    国家自然科学基金面上项目;国家自然科学基金面上项目

Selenite induces apoptosis in colorectal cancer cell line SW480

  • Received:2016-09-18 Revised:2016-10-22 Online:2016-12-05 Published:2016-11-29
  • Supported by:
    the National Natural Science Foundation of China;the National Natural Science Foundation of China

摘要: 目的 探讨亚硒酸钠诱导结直肠癌SW480细胞凋亡的信号通路。 方法 将SW480细胞分为三组,分别进行亚硒酸钠、MnTMPyP以及两者联用处理;用DCFH-DA荧光探针法检测细胞内活性氧;Western blot方法检测细胞信号通路JNK和ATF-2的磷酸化,以及c-Myc,c-Jun,c-Fos,cyclin D1,cyclin A2,Bcl-2等相关蛋白表达;流式细胞术检测SW480细胞凋亡率。 结果 亚硒酸钠可使SW480细胞产生活性氧;抑制JNK和ATF-2的磷酸化(P<0.05),下调c-Myc,c-Jun,c-Fos,cyclin D1,cyclin A2,Bcl-2的表达诱导 SW480细胞凋亡(P<0.05);用活性氧抑制剂MnTMPyP可减轻亚硒酸钠所致的上述变化(P<0.05)。结论 亚硒酸钠可通过产生活性氧,抑制JNK和ATF-2的磷酸化,下调c-Myc, c-Jun, c-Fos, cyclin D1, cyclin A2, Bcl-2的表达,促进SW480细胞的凋亡。

关键词: 亚硒酸钠, SW480细胞, 活性氧, JNK, 细胞周期蛋白

Abstract: Objective To investigate the signaling pathway involved in the colorectal cancer SW480 cell apoptosis induced by selenite. Methods SW480 cells were divided into three groups, respectively treated with selenite, MnTMPyP and their combinations. Monitoring the ROS content in SW480 cells by DCFH-DA fluorescence probes. The phosphorylation of JNK and ATF-2,and the expression of c-Myc,c-Jun,c-Fos,cyclin D1,cyclin A2,Bcl-2 and other related proteins were detected by Western blot. Cell apoptosis rate was detected by flow cytometry. Results Selenite can induce SW480 cells to produce ROS. Selenite can induced SW480 cell apoptosis by downregulating the phosphorylation of JNK and ATF-2,and the expression of c-Myc, c-Jun,c-Fos, cyclin D1, cyclin A2, Bcl-2(P<0.05). The ROS inhibitor MnTMPyP can reduced the effects caused by selenite(P<0.05). Conclusions Selenite can induce ROS generation,inhibit JNK and ATF-2 phosphorylation and the expression of c-Myc,c-Jun,c-Fos,cyclin D1,cyclin A2 and Bcl-2, thus promote apoptosis in SW480 cells.

Key words: selenite, SW480 cell, ROS, JNK, cyclins

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