结直肠癌stat3表达与细胞增殖、凋亡及预后的关系

基础医学与临床 ›› 2016, Vol. 36 ›› Issue (11): 1531-1536.

结直肠癌stat3表达与细胞增殖、凋亡及预后的关系

罗枫¹,伍世钢¹,刘坤平²

1. 广东省清远市人民医院
2. 清远市人民医院

收稿日期:2016-01-11 修回日期:2016-05-01 出版日期:2016-11-05 发布日期:2016-10-24
通讯作者: 刘坤平 E-mail:gdqylkp@163.com
基金资助:
广东省自然科学基金;清远市科技计划项目;清远市科技计划项目

Significance of apoptotic and proliferative indices and stat3 expression in colorectal carcinoma

Received:2016-01-11 Revised:2016-05-01 Online:2016-11-05 Published:2016-10-24
Contact: Liu kunping E-mail:gdqylkp@163.com

摘要/Abstract

摘要： 目的探讨结直肠癌stat3蛋白表达与细胞增殖、凋亡、临床病理特征及预后的关系。方法应用免疫组织化学及DNA末端标记（TUNEL）技术分别检测129例结直肠癌及癌旁组织芯片中stat3、CyclinD1蛋白表达及凋亡指数（AI），分析stat3表达与临床病理特征和预后的关系及其与增殖、凋亡之间的相关性。结果结直肠癌组织中stat3、CyclinD1阳性表达率分别为67.4%和79.8%，均高于癌旁组织的43.4%和5.4%（P＜0.05），结直肠癌组织AI（5.62）低于癌旁（7.86）（P＜0.05）。stat3的表达与结直肠癌的Dukes分期、淋巴结转移、肿瘤发生部位明显相关（P＜0.05）。结直肠癌组织中stat3和CyclinD1的表达呈正相关（P＜0.05）。Kaplan-Meier生存分析显示stat3、Dukes分期、淋巴结转移及凋亡指数与生存期相关（P＜0.05）；Cox回归分析显示Dukes分期为独立的风险预后因子。结论 stat3可能参与了结直肠癌的发生发展，检测stat3可作为评估结直肠癌患者预后及淋巴结转移的指标。

关键词: 结直肠癌, stat3, CyclinD1, 凋亡, 预后

Abstract: Objective To investigate the relationship between apoptosis and cell proliferation and the expression of stat3 as well as their prognostic significance in colorectal carcinoma(CRC). Methods Samples from 129 patients with CRC and adjacent normal mucosa were analyzad for stat3 and CyclinD1 expression with immunohistochemistry, and the apoptotic index with TUNEL method. The association of stat3 expression with clinicopathological parameters, prognosis, apoptotic and proliferative activities was subsequently analyzed. Results The expression of stat3 and CyclinD1 showed significant difference (P＜0.05) between CRC tissues and adjacent non-cancerous tissues. The level of AI in CRC tissues was statistically lower than that in adjacent non-cancerous tissues (P＜0.05).The expression of stat3 was correlated with Dukes staging, lymph node metastasis and tumor location of CRC (P＜0.05).Stat3 expression was positively correlated with CyclinD1 (P＜0.05).Kaplan-Meier univariate analysis revealed stat3 positive expression, Dukes staging, lymph node metastasis and AI had significance for survival time（P＜0.05）. Cox model analysis revealed Dukes staging was a dangerous factor for independent prognosis of CRC. Conclusions Stat3 might participate in the development and progression of colorectal cancer. Stat3 protein can be acted as index to judge lymph node metastases and prognosis assessment of colorectal carcinoma.

Key words: Colorectal cancer, stat3, CyclinD1, apoptosis, prognosis

中图分类号:

R735.3+4

罗枫伍世钢刘坤平. 结直肠癌stat3表达与细胞增殖、凋亡及预后的关系[J]. 基础医学与临床, 2016, 36(11): 1531-1536.

[1]	刘雯霞刘彩红郭蕊孟芝君高佳王亚静. 尼古丁促进高糖/高脂培养的大鼠心肌细胞系H9C2凋亡[J]. , 2020, 40(1): 24-29.
[2]	蒋庆锋于永魁程金华申思宁邢文群. lncRNA HEIH通过miR-98-5p调节肺癌细胞增殖和凋亡[J]. , 2020, 40(1): 30-36.
[3]	苗玉娟王淑坤孙丹丁康刘美逸张妍周风华. miR-181 通过 Prox1 促进人乳腺癌细胞系MCF-7的增殖并抑制凋亡[J]. 基础医学与临床, 2019, 39(9): 1265-1269.
[4]	童静凯江霞. 托伐普坦对小鼠内髓集合管细胞系水通道蛋白及肾小管功能的影响[J]. 基础医学与临床, 2019, 39(8): 1163-1167.
[5]	晋瑞金迎迎李嫚雷喆. 过表达脾酪氨酸激酶降低氧化应激诱导的小鼠成骨细胞凋亡[J]. 基础医学与临床, 2019, 39(7): 1013-1018.
[6]	任玮余宏川王萍刘英茹. miR-17-5p诱导小鼠肾足细胞系凋亡[J]. 基础医学与临床, 2019, 39(7): 973-977.
[7]	刘琦郭毅郭金竹窦万臣. 颞叶海绵状血管瘤继发癫痫的手术治疗[J]. 基础医学与临床, 2019, 39(7): 1040-1044.
[8]	雷晓燕孙永红陈星星崔维静刘璟. 乙肝病毒X蛋白抑制小鼠足细胞系MPC5增殖并促进其凋亡[J]. 基础医学与临床, 2019, 39(5): 617-622.
[9]	马从乾王雅王娜杨柯陈德才. miR-181a过表达加重H2O2诱导的HUVECs损伤[J]. 基础医学与临床, 2019, 39(5): 690-695.
[10]	王圣凯胡苏琴吴玲吴杭菲郑靖宇蒋自培李剑敏. miR-138在乙醇诱导的大鼠心肌细胞系H9C2氧化应激中表达增加[J]. 基础医学与临床, 2019, 39(5): 677-681.
[11]	战仕胜李军杨婷婷王雅蓉王秀青. 抗菌肽merecidin诱导人肺腺癌细胞系A549凋亡[J]. 基础医学与临床, 2019, 39(4): 473-477.
[12]	刘莹张艳丽王秀芳. 沉默FOXO1抑制H2O2诱导的大鼠肺泡2型上皮细胞凋亡[J]. 基础医学与临床, 2019, 39(4): 519-523.
[13]	张娟冯燕和水祥. 过表达miR-5195-3p对结直肠癌细胞凋亡的影响[J]. 基础医学与临床, 2019, 39(3): 385-391.
[14]	张许梅王瑾霍海燕岳继萍张文婷焦向英. 血管紧张素II 1型受体自身抗体可上调TXNIP导致大鼠胰岛β细胞系INS-1凋亡[J]. , 2019, 39(11): 1548-1555.
[15]	魏来张研. APPswe/PS1dE9双转基因小鼠中轴突异常与阿尔茨海默症的联系[J]. , 2019, 39(11): 1525-1529.