基础医学与临床 ›› 2015, Vol. 35 ›› Issue (8): 1067-1071.

• 研究论文 • 上一篇    下一篇

胃癌中YAP与β-catenin蛋白表达的临床意义及相关性

官文飞1,母松2,蒙长远1,张军1   

  1. 1. 重庆医科大学附属第一医院
    2. 重庆医科大学附属第一医院胃肠外科
  • 收稿日期:2014-12-18 修回日期:2015-03-26 出版日期:2015-08-05 发布日期:2015-07-15
  • 通讯作者: 张军 E-mail:zjun2323@yahoo.com.cn
  • 基金资助:
    重庆市科技攻关应用技术研发项目

Clinical significance and correlation of Yes-associated protein and β-catenin expression in gastric cancer

  • Received:2014-12-18 Revised:2015-03-26 Online:2015-08-05 Published:2015-07-15

摘要: 目的 探讨YAP及β-catenin在胃癌组织、癌旁正常组织的表达与临床病理特征的关系及两者表达相关性。方法 采用荧光定量PCR、免疫组化和Western blot测定YAP和β-catenin在50例胃癌组织及癌旁正常组织中mRNA及蛋白表达,并分析其与临床病理参数关系及两者表达的相关性。结果 胃癌组织中YAP和β-catenin蛋白阳性表达率分别为72%(36/50)和64%(32/50),癌旁正常组织中阳性表达率分别为14%(7/50)和20%(10/50),P<0.05;YAP及β-catenin mRNA和蛋白在胃癌组织中表达较正常组织高(P<0.05), 且两者表达水平与胃癌分化程度、淋巴结转移和临床分期有显著关系 (P<0.05);YAP与β-catenin在胃组织中共表达呈明显正相关(r=0.72 ,P<0.05)。结论 Yap和β-catenin蛋白在胃癌组织中存在高表达共存现象,可能与胃癌细胞恶性程度及细胞分化有关,参与胃癌的发生、发展过程。

关键词: 胃肿瘤, Hippo/YAP, Wnt/β-catenin, 信号转导通路

Abstract: Objective To investigate the expressions and correlation of YAP andβ-catenin in the gastric carcinoma(GC) tissues and the paired normal gastric tissues. Methods Fluorescence quantitative PCR(RT-qPCR), immunocytochemistry staining and Western blotting were used to detect the mRNA and protein expressions of YAP and β-catenin in GC tissues and the paired normal gastric tissues,The correlations between the expressions and the clinicopathological characteristics of GC were analyzed statistically. Results The positive expression of YAP and β-catenin were respectively 72% (36/50) and 64% (32/50), and the positive expression of paired normal gastric tissues were 14% (7/50) and 20% (10/50), P<0.05; The mRNA and protein levels of YAP and β-catenin in GC tissues were higher than those in the paired normal tissues(P<0.05).Furthermore, the expression was obviously correlated to cell differentiation, lymph node metastases and clinical stage of GC( P< 0.05). The significantly positive correlation was found between the expressions of YAP and β-catenin in GC tissues(r=0.72, P<0.05). Conclusion The high expression of YAP and β-catenin coexist in GC tissues. It may be related to malignant transformation and abnormal proliferation of cells, which facilitates the occurrence and development of GC.

Key words: stomach neoplasm, Yes-associated protein, β-catenin, signal transduction pathway

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