基于IPA@生物信息平台筛选维吾尔族宫颈癌前病变患者血浆预警蛋白

基础医学与临床 ›› 2014, Vol. 34 ›› Issue (1): 6-10.

基于IPA@生物信息平台筛选维吾尔族宫颈癌前病变患者血浆预警蛋白

郭霞¹,伊力努尔?沙比提²,玛依拉?卡米力江²,刘开江³,阿仙姑?哈斯木²,哈丽丹?热依木²,武贵臻¹,Abulizi Abudula²

1. 新疆医科大学新疆地方病分子生物学重点实验室
2. 新疆医科大学
3. 新疆医科大学第三附属肿瘤医院妇科

收稿日期:2013-03-08 修回日期:2013-05-03 出版日期:2014-01-05 发布日期:2013-12-26
通讯作者: 郭霞 E-mail:myshow0504@163.com
基金资助:
宫颈癌前病变的HPV感染、血清抗体免疫应答和血清蛋白标志物研究;维吾尔族妇女宫颈癌及HPV感染共性血清蛋白指标的特异性、灵敏度和通用性验证

Screening the potential early-warning plasma biomarkers specific to cervical precancerous lesion in Uyghur women based on IPA@ bioinformatics analysis

Received:2013-03-08 Revised:2013-05-03 Online:2014-01-05 Published:2013-12-26

摘要/Abstract

摘要： 目的依托IPA@在线生物信息学平台探讨维吾尔族患者宫颈癌前病变的发生与癌变机理，寻找血浆预警蛋白。方法运用IPA@在线生物信息学软件，针对前期研究中通过蛋白质组学方法筛选出的在维吾尔族妇女癌前病变宫颈内上皮瘤样病变CINⅡ和Ⅲ级患者血浆中31个差异蛋白进行生物学功能注释、调控网络分析、典型通路分析和生物标志物筛查分析。结果 IPA@分析发现CINⅡ/Ⅲ患者血浆中的差异蛋白的生物功能主要集中在炎症反应、细胞与细胞之间信号与作用和细胞生长与增殖，涉及的通路主要是急性期反应通路、JAK/Stat 通路和IL-4通路，并且筛选出2种生物标志性蛋白，它们是代谢相关蛋白（ApoAⅠ）、信号转导相关蛋白(mTOR)。结论 ApoAⅠ和mTOR可作为宫颈癌及癌前病变的候选血浆预警蛋白。

关键词: 维吾尔族妇女, 宫颈内上皮瘤样病变, IPA@生物信息学分析, 血浆生物标志物

Abstract: Objective This study aimed to screen the early-warning plasma biomarkers of cervical intraepithelial neoplasiaⅡ/Ⅲ stage (CINⅡ/Ⅲ) in Uygur women by using Ingenuity Pathway Analysis (IPA), as well as explain the canceration mechanism of cervical cancer. Methods 31 plasma differentially expressed proteins which were analyzed and identified by proteomic techniques were performed by functional annotation, networks analysis, biofuction analysis, canonical pathways analysis and IPA–biomarker? filter analysis using IPA@ online bioinformatics software. Results As the result of IPA@ analysis, when classified according to function, inflammatory response, cell to cell signaling and interaction, cellular growth and proliferation were most frequently identified in CINⅡ/Ⅲ. Acute phase response signaling and JAK/Stat signaling and IL-4 signaling,etc were identified as the canonical pathways that are overrepresented in CINⅡ/Ⅲ. Two plasma proteins (ApoAⅠ and mTOR) as candidate biomarker were screened. Conclusion The proteins ApoAⅠand mTOR could considered as candidated plasma markers of cervical cancer and cervical precancerous lesion.

Key words: Uyghur women, cervical intraepithelial neoplasia, IPA@ bioinformatics analysis, plasma biomarkers.

中图分类号:

R917

郭霞伊力努尔?沙比提玛依拉?卡米力江刘开江阿仙姑?哈斯木哈丽丹?热依木武贵臻 Abulizi Abudula. 基于IPA@生物信息平台筛选维吾尔族宫颈癌前病变患者血浆预警蛋白[J]. 基础医学与临床, 2014, 34(1): 6-10.