溶血磷脂酸调控RhoA/ROCK2信号通路对乳腺癌细胞增殖的影响

基础医学与临床 ›› 2013, Vol. 33 ›› Issue (1): 106-110.

溶血磷脂酸调控RhoA/ROCK2信号通路对乳腺癌细胞增殖的影响

许海¹,段刚峰²

1. 湖北中医药大学黄家湖医院
2. 武汉市第一医院

收稿日期:2012-06-26 修回日期:2012-10-25 出版日期:2013-01-05 发布日期:2012-12-25
通讯作者: 许海 E-mail:643025@qq.com

Influence of lysophosphatidic acid on proliferation of breast cancer cell by adjusted RhoA/ROCK2 signal pathway

Received:2012-06-26 Revised:2012-10-25 Online:2013-01-05 Published:2012-12-25

摘要/Abstract

摘要： 目的探讨溶血磷脂酸(LPA)与RhoA/ROCK2信号通路对乳腺癌细胞增殖的影响及其作用机制。方法以不同浓度LPA干预乳腺癌MDA-MB-231细胞，每隔24h以细胞计数法观察和记录细胞的增殖。以最佳LPA促增殖浓度作用于MDA-MB-231细胞，观察Rho激酶抑制剂(Y-27632)对癌细胞的影响；以Pull-down及Western blot法检测各组细胞内RhoA活性及RhoA、ROCK2蛋白表达。结果 LPA以时间及剂量依赖性关系显著促进MDA-MB-231细胞的增殖(P<0.05)；Y-27632可以显著抑制LPA的促增殖作用；LPA干预后RhoA活性及RhoA、ROCK2蛋白表达显著升高(P<0.05)，Y-27632干预后RhoA活性及RhoA、ROCK2蛋白表达显著下降(P<0.05)。结论 LPA可能通过调控RhoA/ROCK2信号通路促进乳腺癌细胞的增殖，为乳腺癌的临床治疗提供了新思路。

关键词: 溶血磷脂酸, 乳腺癌, 增殖, RhoA/ROCK2信号通路

Abstract: Objective To investigate the influence and mechanism of lysophosphatidic acid and RhoA/ROCK2 signal pathway on proliferation of breast cancer cell. Methods After breast cancer cell(MDA-MB-231) was treated with different concentration of LPA, the proliferation of MDA-MB-231 was observed and recorded by cell count method every of 24h. MDA-MB-231 was treated with optimal concentration of LPA and observed the effect of Rho kinase inhibitor(Y-27632) on LPA-induced proliferation. The activity of RhoA was tested by Pull-down way. The protein expression of RhoA and ROCK2 were determined by Western blot. Results LPA could promote MDA-MB-231 proliferation in a time and dose-dependent manner (P<0.05). ROCK inhibitor could significantly inhibit LPA-induced cell proliferation(P<0.05). The activity of RhoA and expressionof RhoA, ROCK2 were enhanced significantly affected by LPA (P<0.05). However Y-27632 markedly decreased LPA-induced the increase of RhoA activity and protein expression of RhoA and ROCK2 (P<0.05). Conclusions LPA may promote breast cancer cell proliferation through regulating by RhoA/ROCK2 signal pathway. It provides a new idea for breast cancer clinical treatment.

Key words: lysophosphatidic acid, breast cancer, proliferation, RhoA/ROCK2 signal pathway

许海段刚峰. 溶血磷脂酸调控RhoA/ROCK2信号通路对乳腺癌细胞增殖的影响[J]. 基础医学与临床, 2013, 33(1): 106-110.

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