埃博拉病毒包膜蛋白GP基因DNA疫苗诱导小鼠产生高滴度中和抗体

基础医学与临床 ›› 2011, Vol. 31 ›› Issue (6): 667-671.

埃博拉病毒包膜蛋白GP基因DNA疫苗诱导小鼠产生高滴度中和抗体

张桂根,郎建社,王洪亮,刘康泰,蒋澄宇

中国医学科学院基础医学研究所北京协和医学院基础学院

收稿日期:2011-03-22 修回日期:2011-03-30 出版日期:2011-06-05 发布日期:2011-06-06
通讯作者: 蒋澄宇 E-mail:chengyujiang@gmail.com
基金资助:
国家自然科学基金

DNA vaccine encoding Ebola virus envelope glycoprotein inducing high-titer neutralization antibody in mice

Gui-gen ZHANG¹,Jian-she LANG²,Hong-liang WANG²,Kang-tai LIU²,Cheng-yu JIANG¹

1. Institute of Basic Medical Sciences, CAMS & PUMC
2.

Received:2011-03-22 Revised:2011-03-30 Online:2011-06-05 Published:2011-06-06
Contact: Cheng-yu JIANG E-mail:chengyujiang@gmail.com
Supported by:
National Natural Science Foundation of China

摘要/Abstract

摘要： 目的探讨埃扎伊尔-埃博拉病毒（Zaire ebolavirus）包膜蛋白（GP）DNA疫苗诱导小鼠产生中和抗体的特性及研制埃博拉病毒DNA疫苗的可行性。方法构建埃博拉病毒包膜蛋白GP真核表达质粒Peak13CD5LGP，采用100?g剂量的质粒免疫小鼠，以纯化的埃博拉病毒包膜蛋白亚单位融合蛋白（GP1-Fc）作为抗原，测定血清抗体滴度及特异性。结果100?g质粒免疫小鼠三次后，通过ELISA检测及Western blot验证，小鼠血清中GP中和抗体滴度达到1：2000。结论含埃博拉病毒包膜蛋白GP基因的DNA疫苗（pEAK13CD5LGP）能够诱导小鼠产生高滴度和持久的中和抗体，作为埃博拉病毒疫苗具有潜在的应用价值。

关键词: 埃博拉病毒, 包膜蛋白, DNA疫苗

Abstract: Obejective To study the characteristics of neutralization antibody in mice induced by DNA vaccine encoding Zaire ebolavirus glycoprotein (GP) and develop DNA vaccine against Ebola virus. Methods Zaire Ebolavirus GP encoding plasmid Peak13CD5LGP was constructed. 100 ?g dosage of Peak13CD5LGP was used to immunize the mice, the titer of neutralization antibody against Ebola Zaire virus glycoprotein was assayed by ELISA using Ebola Zaire virus glycoprotein subunit GP1-Fc fusion protien as capture antigen. Results After boost immunization with 100 ?g dosage Peak13CD5LGP plasmid, the titer of neutralization antibody of anti-GP was 1:2000, and both efficiency and specificity of neutralization antibody was verified by western blot. Conlusion 100?g dosage of DNA vaccine encoding Zaire Ebolavirus envelope glycoprotein is able to induce persistent and high level of neutralizing anibody, and may be potential valuable vaccine against the deadly Zaire Ebolavirus.

Key words: Zaire Ebolavirus, envelope glycoprotein, DNA vaccine

中图分类号:

R373.9

张桂根郎建社王洪亮刘康泰蒋澄宇. 埃博拉病毒包膜蛋白GP基因DNA疫苗诱导小鼠产生高滴度中和抗体[J]. 基础医学与临床, 2011, 31(6): 667-671.

Gui-gen ZHANG Jian-she LANG Hong-liang WANG Kang-tai LIU Cheng-yu JIANG. DNA vaccine encoding Ebola virus envelope glycoprotein inducing high-titer neutralization antibody in mice[J]. Basic & Clinical Medicine, 2011, 31(6): 667-671.

[1]	朱江高蕾. 重组腺相关病毒表达干扰素α体外抑制乙肝病毒的复制[J]. 基础医学与临床, 2015, 35(10): 1331-1335.
[2]	何艳王丹丹陈华妹李勇杰薛启祥王旭东. 雌激素通过ERK-FAK信号通路调节MCF-7乳腺癌细胞失巢凋亡[J]. 基础医学与临床, 2015, 35(9): 1194-1198.
[3]	刘晓红王筑婷李阳杨莉雷霆雯王旭东. 钙激活中性蛋白酶介导雌激素增强MCF-7乳腺癌细胞的存活力[J]. 基础医学与临床, 2013, 33(7): 799-803.
[4]	杨莉朱筑霞刘晓红胡晓霞王红梅王旭东. 钙蛋白酶在乳腺上皮转化细胞对雌激素刺激反应中的作用[J]. 基础医学与临床, 2013, 33(7): 819-823.
[5]	彭清林张婷范东升郑伟谢喜秀许于飞许雪梅. HPV 18 L1病毒样颗粒的表达纯化及其豚鼠抗血清制备[J]. 基础医学与临床, 2009, 29(10): 1039-1043.