基础医学与临床 ›› 2011, Vol. 31 ›› Issue (2): 166-169.

• 研究论文 • 上一篇    下一篇

人精浆中性α-1,4-糖苷酶活性与精子透明质酸酶活性的关系

马晓萍1,高晓勤2,丁贤胜3,倪佳3   

  1. 1. 贵阳医学院 组织胚胎学教研室
    2. 遵义医药高等专科学校
    3. 贵阳医学院
  • 收稿日期:2010-02-25 修回日期:2010-07-02 出版日期:2011-02-05 发布日期:2011-03-14
  • 通讯作者: 高晓勤 E-mail:gxq550301@sina.com
  • 基金资助:
    黔科合S字(2007)1030];省科技发展项目

Relationship between seminal plasma neutral alpha-1,4 glycosidase activity and the positive rate and activity intensity of hyaluronidase in infertile males

MA Xiao-ping 1,GAO Xiao-qin 2,DING Xian-sheng 3,NI Jia 3   

  1. 1. Guiyang Medical College
    2. Zunyi Medical and Pharmaceutical College
    3.
  • Received:2010-02-25 Revised:2010-07-02 Online:2011-02-05 Published:2011-03-14
  • Contact: GAO Xiao-qin E-mail:gxq550301@sina.com

摘要: 目的 探讨人精浆中性α-1,4-糖苷酶(中性α-1,4-G)活性与精子透明质酸酶(HYD)活性(HYD阳性反应率及HYD活性强度)的关系。方法 收集年龄24~40岁不育男性精液38例,正常对照12例,根据精浆中性α-1,4-G活性检测结果分为2组:精浆中性α-1,4-G活性正常组(26例)与精浆中性α-1,4-G活性异常组(12例)。24~40岁正常生育男性精液12例作为对照组。用检测盒测定中性α-1,4-G活性,用改良固定底物膜法分别检测精子顶体内HYD阳性反应率及HYD活性强度。结果 不育各组中的精浆中性α-1,4-G活性和精子HYD活性(HYD阳性反应率、HYD活性强度)均显著低于正常生育组(P<0.01)。精浆中性α-1,4-G活性与HYD活性(HYD阳性反应率、HYD活性强度)呈显著正相关(r值分别为0.877,0.910;P值均小于0.01)。结论 精浆中性α-1,4-G活性对精子HYD活性(HYD阳性反应率、HYD活性强度)有明显影响。

关键词: 精浆中性a一1,4一糖苷酶, 透明质酸酶, 男性不育

Abstract: Objective To study the relationship between neutral alpha—1,4 glycosidase(α-1,4-G )activity of human seminal plasma and the positive rate and activity intensity of hyaluronidase(HYD) .Method 1.To collect 38 infertile men’s semen, who were between 24 and 40 years old and be devided into 2 groups according to the result of neutral α-1,4-G activity test. They were normal neutral α-1,4-G activity group(26 cases) and abnormal α-1,4-G activity group(12 cases). Normal birth masculine seminal fluid matched ages was the control group (12cases). Neutral α-1,4-G activity in semen test kits was used to detect the neutral α-1,4-G activity . The positive rate and activity intensity of HYD in 2 groups were examined by our improved fixed—substrate film method. Results There was significant difference between normal birth group and infertile groups(P<0.01). The relation between neutral α-1,4-G activity and the positive rate of HYD had a significant positive correlation (r=0.877,P<0.01); The relation between neutral α-1,4-G activity and the activity intensity of HYD showed a significant positive correlation (r=0.910,P<0.01). Conclusion The neutral α-1,4-G activity of human seminal plasma could affect the positive rate and activity intensity of HYD.

中图分类号: