基础医学与临床 ›› 2011, Vol. 31 ›› Issue (12): 1394-1395.

• 研究短文 • 上一篇    下一篇

老年脑梗塞患者核因子-кB和单核细胞趋化蛋白-1的关系及其在动脉粥样硬化中的作用

黄建敏1,黄瑞雅2,简崇东2,李雪斌2,蒙兰青2,唐雄林2   

  1. 1. 广西右江民族医学院附属医院
    2.
  • 收稿日期:2010-10-28 修回日期:2011-04-12 出版日期:2011-12-05 发布日期:2011-12-05
  • 通讯作者: 黄建敏 E-mail:bshuangjianmin@126.com

Relationship between nuclear factor-kappa B and monocyte chemoattractant protein-1 in elderly patients with cerebral infarction and their roles in atherosclerosis

  • Received:2010-10-28 Revised:2011-04-12 Online:2011-12-05 Published:2011-12-05

摘要: 摘要:目的 探讨老年脑梗塞患者核因子-кB(NF-кB)和单核细胞趋化蛋白-1(MCP-1)的关系及其在动脉粥样硬化中的作用。方法 通过DSA检查确诊颈动脉狭窄的老年脑梗塞患者72例作为研究对象(脑梗塞组,其中轻度狭窄33例,中度狭窄24例,重度狭窄15例),以非脑血管病健康体检者38例作为对照组,用TransAM试剂盒检测NF-кB DNA结合活性,用双抗体夹心酶联免疫吸附法检测MCP-1水平,分析NF-кB与MCP-1、血糖和血脂的关系。结果 脑梗塞组患者NF-кB活性和MCP-1水平均显著高于对照组(均P<0.01),各狭窄组患者NF-кB活性和MCP-1水平均有显著性差异(均P<0.01),并且随颈动脉狭窄程度加重NF-кB活性和MCP-1水平均呈上升趋势,相关分析显示,脑梗塞组患者NF-кB活性与MCP-1水平呈正相关(r=0.72,P<0.01),与血糖水平呈正相关(r=0.58,P<0.01)。结论 NF-кB和MCP-1及其复杂的相互作用参与了动脉粥样硬化炎症反应机制,同时测定NF-кB和MCP-1对评估脑梗塞的危险性具有重要的意义。

关键词: 脑梗塞, 老年, 核因子-кB, 单核细胞趋化蛋白-1, 动脉粥样硬化

Abstract: Abstract: Objective To investigate the relationship between nuclear factor-kappa B( NF-кB ) and monocyte chemoattractant protein-1( MCP-1) in elderly patients with cerebral infarction and their roles in atherosclerosis. Methods By DSA examination, 72 elderly patients with cerebral infarction combined carotid artery stenosis were involved in the study. The light stenosis was seen in 33 cases, the middle stenosis was seen in 24 cases, the heavy stenosis was seen in 15 cases. 38 healthy people without Cerebrovascular Disease were adopted as control group. NF-кB DNA binding activity was measured by Trans- AM transcription factor assay kits . The levels of serum MCP-1 were detected by ELISA. We have analyzed correlation between NF-кB, MCP-1, blood glucose and blood fat. Results The activity of NF-кB and the levels of serum MCP-1 in the cerebral infarction group were significantly higher than those in the control group( P<0.01). There are significant difference in the activity of NF-кB and the levels of serum MCP-1 in the different stenosis groups( P<0.01). The activity of NF-кB and the levels of serum MCP-1 in cerebral infarction group increased with the progression of carotid artery stenosis. The correlation analysis results showed that there was positive correlation between NF-кB and MCP-1(r=0.72,P<0.01),and there was also positive correlatio between NF-кB and blood glucose,(r=0.58,P<0.01) in cerebral infarction groups. Conclusion The results indicate that the complicated interaction between NF-кB and MCP-1 may involved in inflammatory reaction mechanism of atherosclerosis. The simultaneous determination of NF-кB and MCP-1 could have am important significance to evaluate risk of cerebral infarction.

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