罗格列酮上调高脂饮食老年大鼠骨骼肌CPTⅠ和PPARγ的表达

基础医学与临床 ›› 2010, Vol. 30 ›› Issue (9): 935-939.

罗格列酮上调高脂饮食老年大鼠骨骼肌CPTⅠ和PPARγ的表达

高宇马慧娟王敬曲东明宋光耀胡淑国

承德医学院附属医院河北省人民医院河北省人民医院河北省人民医院河北省人民医院

收稿日期:2009-09-11 修回日期:2010-01-06 出版日期:2010-09-05 发布日期:2010-09-05
通讯作者: 宋光耀

Rosiglitazone up-regulates expression of CPTⅠand PPARγ in skeletal muscles of aged rat fed high-fat diets

Yu GAO, Hui-juan MA, Jing WANG, Dong-ming QU, Guang-yao SONG, Shu-guo HU

Affiliated Hospital, Chengde Medical College Hebei Provincial People's Hospital Hebei Provincial People's Hospital

Received:2009-09-11 Revised:2010-01-06 Online:2010-09-05 Published:2010-09-05
Contact: Guang-yao SONG,

摘要/Abstract

摘要： 目的观察高脂饮食对老年大鼠骨骼肌肉碱棕榈酰基转移酶Ⅰ（CPTⅠ）和过氧化体增殖物激活受体γ（PPARγ）的表达变化及罗格列酮的干预效果。方法大鼠随机分为对照组、高脂组（HF）、罗格列酮干预组（RSG）,每组20只。应用清醒状态下正常葡萄糖高胰岛素钳夹技术的葡萄糖输注率评价胰岛素抵抗，用RT-PCR法和Western blot印迹技术检测骨骼肌CPTⅠ和PPARγ的表达。结果高脂组骨骼肌甘油三酯（TG）升高而葡萄糖输注率明显下降（P<0.01），骨骼肌CPTⅠ和PPARγ表达明显降低(CPTⅠb mRNA 3.16±0.59vs1.30±0.18) (PPARγmRNA1.48±0.25vs1.04±0.33)（P<0.05, P<0.01），罗格列酮干预组显著缓解高脂组上述变化(CPTⅠb mRNA1.30±0.18vs2.84±0.72) (PPARγmRNA1.04±0.33vs2.13±0.42)（P<0.01）。结论罗格列酮上调高脂饮食老年大鼠骨骼肌CPTⅠ和PPARγ的表达，可能是减少骨骼肌内脂质堆积改善胰岛素抵抗的机制之一。

关键词: 老年, 胰岛素抵抗, 肉碱棕榈酰基转移酶Ⅰ, 过氧化物酶体增殖物激活受体γ, 罗格列酮

Abstract: Objective To observe the expression of carnitine palmitoyltransferase Ⅰ (CPTⅠ) and peroxisome proliferators activated receptorγ(PPARγ) in high-fat-fed aged rats and rosiglitazone intervention. Methods Wistar rats were divided into control group, high-fat diet (HF) group and high-fat diet plus rosiglitazone maleate tablets (RSG) group, (n=20 in each group). Insulin sensitivity was evaluated by conscious hyperinsulinemic-euglycemic clamp techniques, the expression of CPTⅠmRNA and PPARγmRNA and protein were analyzed by RT-PCR and Western-blot. Results Skeletal muscle triglyceride was significantly higher and glucose infusion rate was significantly lower in the control group than those in the high-fat diets group. The expression of CPTⅠ and PPARγ decreased in HF group, RSG group reserves above of all the changes. Conclusion It is suggested that a increased expression of CPTⅠand PPARγ play a pivotal role in insulin-sensitizing effect of rosiglitazone treatment, maybe the one of the mechanism of improving insulin resistance.

Key words: Aging, Insulin resistance, Carnitine palmitoyltransferase（CPTⅠ）, PPAR gamma, Rosiglitazone

高宇马慧娟王敬曲东明宋光耀胡淑国. 罗格列酮上调高脂饮食老年大鼠骨骼肌CPTⅠ和PPARγ的表达[J]. 基础医学与临床, 2010, 30(9): 935-939.

Yu GAO; Hui-juan MA; Jing WANG; Dong-ming QU; Guang-yao SONG; Shu-guo HU. Rosiglitazone up-regulates expression of CPTⅠand PPARγ in skeletal muscles of aged rat fed high-fat diets[J]. Basic & Clinical Medicine, 2010, 30(9): 935-939.

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