慢性乙肝患者外周血单个核细胞TRAIL受体表达及其临床意义

基础医学与临床 ›› 2010, Vol. 30 ›› Issue (3): 275-279.

慢性乙肝患者外周血单个核细胞TRAIL受体表达及其临床意义

冷向锋陈振雨张秋孙汶生

青岛大学医学院附属医院山东大学化学与化工学院山东大学医学院免疫学研究所

收稿日期:2009-06-29 修回日期:2009-09-16 出版日期:2010-03-05 发布日期:2011-05-04
通讯作者: 张秋

Expression of tumor necrosis factor related apoptosis inducing ligand ( TRAIL) receptors on peripheral blood mononuclear cells in human chronic Hepatitis B patients and its clinical significance

Xiang-feng LENG, Zhen-yu CHEN, Qiu ZHANG, Wen-sheng SUN

the Affiliated Hospital of Medical College, Qingdao University School of Chemistry and Chemical Engineering, Shandong University School of Medicine, Shandong University

Received:2009-06-29 Revised:2009-09-16 Online:2010-03-05 Published:2011-05-04
Contact: Qiu ZHANG,

摘要/Abstract

摘要： 目的　探索慢性乙肝患者外周血单个核细胞（PBMC）肿瘤细胞坏死因子相关的凋亡诱导配体( TRAIL)受体在PBMC凋亡中的作用，及其与机体肝脏损伤的相关性。方法　用RT-PCR和流式细胞术检测55例慢性乙肝病患者（包括轻度、中度、重度）外周血单个核细胞TRAIL各受体（DR4、DR5、DcR1 和DcR2）表达水平, 同时检测肝功能相关指标, 并进行相关性分析。以30例正常人作为对照。结果　诱骗受体DcR1在慢性乙肝患者的表达显著低于对照组。DcR1的表达随慢性乙肝病情的加重而逐渐降低。在慢性乙肝患者，DcR1的表达与转氨酶（ALT）呈显著负相关，与血清白蛋白成显著正相关。结论　慢性乙肝患者外周血单个核细胞DcR1表达下调可能是其细胞凋亡增加的机制之一，且DcR1表达情况可从一定程度上反映肝脏的损伤程度。

Abstract: Objective To explore the role of the expression of TRAIL receptors on peripheral blood mononuclear cell (PBMC) in the apoptosis of PBMC , and the relativity of the expression of TRAIL receptors with the severity of liver damage in chronic Hepatitis B patients. Methods The expressions of DR4、DR5、DcR1 and DcR2 in 55 cases of chronic hepatitis B patients and 30 cases of normal people were assayed by RT-RCR and Flowcytometery. The relativity of the expression of TRAIL receptors and the severity of liver damage were analized. Results The level of DcR1 in the PBMC of chronic B hepatitis patients was much lower than that of control group (p<0.05). There was close relation between the expression of DcR1 and liver damage (p<0.05). The level of DcR1 was negatively correlated with ALT, and positively correlated with serum albumin. Conclusion The expression level of DcR1 on PBMC in chronic hepatitis B patients was down-regulated, which might contribute to the increased apoptosis of PBMC in chronic B hepatitis patients. The expression of DcR1 can reflect the degree of liver injury in chronic hepatitis B patients to some degree.

Key words: Tumor necrosis factor related apoptosis inducing ligand, Receptor, Hepatitis B virus, Infection

冷向锋陈振雨张秋孙汶生. 慢性乙肝患者外周血单个核细胞TRAIL受体表达及其临床意义[J]. 基础医学与临床, 2010, 30(3): 275-279.

Xiang-feng LENG; Zhen-yu CHEN; Qiu ZHANG; Wen-sheng SUN. Expression of tumor necrosis factor related apoptosis inducing ligand ( TRAIL) receptors on peripheral blood mononuclear cells in human chronic Hepatitis B patients and its clinical significance[J]. Basic & Clinical Medicine, 2010, 30(3): 275-279.

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