二甲基亚砜体外诱导大鼠骨髓间充质干细胞分化为心肌样细胞

基础医学与临床 ›› 2009, Vol. 29 ›› Issue (9): 938-942.

二甲基亚砜体外诱导大鼠骨髓间充质干细胞分化为心肌样细胞

王海萍张雷邵素霞赵静

河北北方学院河北医科大学组织学胚胎学教研室

收稿日期:2008-09-03 修回日期:2008-11-17 出版日期:2009-09-20 发布日期:2009-09-20

Dimethyl sulfoxide induced bone marrow mesenchymal stem cells differentiate into cardiomyocyte-like cells in vitro

Hai-ping WANG, Lei ZHANG, Su-xia SHAO, Jing ZHAO

Hebei North University

Received:2008-09-03 Revised:2008-11-17 Online:2009-09-20 Published:2009-09-20

摘要/Abstract

摘要： 目的探索二甲基亚砜（DMSO）体外诱导大鼠骨髓间充质干细胞（MSCs）向心肌样细胞分化的方法。方法取SD大鼠四肢骨骨髓，分离培养MSCs，应用终浓度为1.0%的DMSO定向诱导，相差显微镜观察细胞形态学变化，应用免疫细胞化学、激光扫描共聚焦显微镜技术检测结蛋白（desmin），α-横纹肌肌动蛋白（α-sarcomeric actin）、心肌特异性肌钙蛋白（C-TnT）的表达，透射电镜观察超微结构。结果 MSCs经体外诱导后分化的细胞均阳性表达desmin、α-sarcomeric actin、C-TnT。透射电镜下可见到平行排列的肌丝和丰富的粗面内质网。结论大鼠骨髓间充质干细胞体外在DMSO诱导下可以定向分化为心肌样细胞。

关键词: 骨髓间充质干细胞, 二甲基亚砜, 心肌细胞, 大鼠

Abstract: Objective To explore the optimal condition for the differentiation of rat bone marrow mesenchymal stem cells(MSCs) into cardiomyocyte-like cells in vitro by using DMSO.Methods SD rat MSCs were isolated from rat bone marrow and cultured, then induced by 1.0%DMSO for 24 h. The cultured cells were observed under phase-contrast microscope. The immunohistochemical technique and laser scanning confocal microscope (LSCM) were used for detecting the expression of desmin, α-sarcomeric actin and C-TnT. The ultrastructures were viewed with transmission electron microscopy.Results MSCs induced by DMSO could be identified by the positive staining for Desmin, α-Sarcomeric Actin and C-TnT.The number of cells stained positively in the 1.0% was visually larger than that of other groups. Transmission electron microscope showed that paralleled myofilaments and a lot of rough endoplasmic reticulum were formed. Conclusion MSCs could be induced to differentiate into cardiomyocyte-like cells by DMSO.

Key words: marrow mesenchymal stem cells, dimethyl sulfoxide, cardiomyocytes, Rat

王海萍张雷邵素霞赵静. 二甲基亚砜体外诱导大鼠骨髓间充质干细胞分化为心肌样细胞[J]. 基础医学与临床, 2009, 29(9): 938-942.

Hai-ping WANG; Lei ZHANG; Su-xia SHAO; Jing ZHAO. Dimethyl sulfoxide induced bone marrow mesenchymal stem cells differentiate into cardiomyocyte-like cells in vitro[J]. Basic & Clinical Medicine, 2009, 29(9): 938-942.

[1]	李贵明王彪方蒡何旭李燕. 谷氨酰胺上调2型糖尿病大鼠结肠组织GLP-1表达[J]. 基础医学与临床, 2019, 39(6): 887-889.
[2]	张源洲席雨鑫温馨李鸿珠. 硫化氢对衰老缺血心肌细胞保护作用的研究进展[J]. 基础医学与临床, 2019, 39(2): 252-255.
[3]	赵承承陈建泉吕纯业. 转染miRNAs降低脂多糖诱导的大鼠肠微血管内皮细胞水通道蛋白1表达[J]. 基础医学与临床, 2019, 39(1): 42-46.
[4]	陈晓依王浩宇吕洋王海萍孙微李柔. PFT-α与BMP-2联合诱导大鼠BMMSCs分化为心肌样细胞[J]. 基础医学与临床, 2018, 38(8): 1088-1093.
[5]	胡慧吴强田茂波. 转染携带Ifi204基因的慢病毒可上调大鼠主动脉成纤维细胞的p204表达[J]. 基础医学与临床, 2018, 38(2): 158-162.
[6]	吴庆程阔菊姚浩旗黄河杨天德. 芒果苷对原代大鼠心肌细胞缺血缺氧凋亡的影响[J]. 基础医学与临床, 2018, 38(2): 169-173.
[7]	赖星宇朱静田杰易勤陈雪妮. Akt在Islet-1促C3H10T1/2向心肌细胞特异分化过程中的作用[J]. 基础医学与临床, 2018, 38(1): 13-19.
[8]	崔桂丽王瑞玲马琴闫佳敏王亚静. 自噬在高糖高脂引起H9C2心肌细胞系凋亡中的影响[J]. 基础医学与临床, 2017, 37(12): 1699-1705.
[9]	王记陆玉琴徐鑫纪召娟. 抑瘤素M促进小鼠诱导多能干细胞向心肌分化[J]. 基础医学与临床, 2017, 37(10): 1401-1406.
[10]	刘振李硕刘玲玲郭志坤王鹏飞. c-kit与microRNA-21在心衰大鼠心肌中表达下降[J]. 基础医学与临床, 2017, 37(1): 76-79.
[11]	王越朱静田杰许皓易勤赖星宇. Islet-1诱导成纤维细胞C3H10T1/2向心肌细胞分化过程中组蛋白乙酰化与DNA甲基化在GATA4启动子区的关系[J]. 基础医学与临床, 2016, 36(9): 1179-1186.
[12]	李丽娜孙伟铭高君王跃虹魏璨徐长庆李鸿珠. 外源性H2S恢复老龄大鼠心肌缺血后适应对I/R损伤的缓解作用[J]. 基础医学与临床, 2016, 36(9): 1199-1205.
[13]	蔚丹丹李亚青李小丽曹静汲振余张明智索振河. 构建OGDH基因敲除型SD大鼠模型[J]. 基础医学与临床, 2016, 36(8): 1068-1073.
[14]	余海军孙善全马岚王富贵. 减压对大鼠脊髓压迫性损伤后脱髓鞘病变和BDNF表达的影响[J]. 基础医学与临床, 2016, 36(5): 633-637.
[15]	陈焱何顺舟李晓莉曾迪丁璐谢江徽季滨龙郑强荪. 静电纺丝聚己内酯纳米纤维支架支持小鼠诱导多能干细胞表达心肌细胞标志物[J]. 基础医学与临床, 2016, 36(4): 486-491.